Prevalence and genetic characteristics of Staphylococcus aureus CC398 isolates from invasive infections in spanish hospitals, focusing on the livestock-independent CC398-MSSA clade

Mama, OM ; Aspiroz, C ; Ruiz-Ripa, L ; Ceballos, S ; Iñiguez-Barrio,M ; Cercenado, E ; Azcona, JM ; López-Cerero, L ; Seral, C (Universidad de Zaragoza) ; López-Calleja, AI ; Belles-Belles, A ; Berdonces, P ; Siller, M ; Zarazaga, M ; Torres, C. ; Leiva, José ; Ezpeleta, Carmen ; Torroba,José Luis ; Castillo, Javier (Universidad de Zaragoza) ; Robres , Pilar ; Torres, Luis ; Alonso, Carla Andrea ; García, Mercé ; Navarro, María ; Vilamala, Anna ; Canut, Andrés ; Aguirre, Amaia ; Megías, Gregoria ; Ayarza, Rafael ; Gomáriz, María ; Calvo, Jorge ; Rezusta, Antonio (Universidad de Zaragoza) ; Palacian, Pilar

Resumen: Background: Livestock-associated (LA)-CC398-MRSA is closely related to pigs, being unfrequently detected in human invasive infections. CC398-MSSA is emerging in human invasive infections in some countries, but genetic and epidemiological characteristics are still scarcely reported.

Objectives: To determine the prevalence of Staphylococcus aureus (SA) CC398, both MRSA and MSSA, among blood cultures SA isolates recovered in Spanish hospitals located in regions with different pig-farming densities (PD) and characterize the recovered isolates.

Methods: One thousand twenty-two SA isolates (761 MSSA, 261 MRSA) recovered from blood cultures during 6–12 months in 17 Spanish hospitals (2018–2019) were studied. CC398 lineage identification, detection of spa-types, and antibiotic resistance, virulence and human immune evasion cluster (IEC) genes were analyzed by PCR/sequencing.

Results: Forty-four CC398-MSSA isolates (4.3% of SA; 5.8% of MSSA) and 10 CC398-MRSA isolates (1% of SA; 3.8% of MRSA) were detected. Eleven spa-types were found among the CC398-MSSA isolates with t571 and t1451 the most frequent spa-types detected (75%). Most of CC398-MSSA isolates were Immune-Evasion-Cluster (IEC)-positive (88.6%), tetracycline-susceptible (95.5%) and erythromycin/clindamycin–inducible-resistant/erm(T)-positive (75%). No statistical significance was detected when the CC398-MSSA/MSSA rate was correlated to PD (pigs/km2) (p = 0.108). On the contrary, CC398-MRSA isolates were all IEC-negative, predominately spa-t011 (70%), and the CC398-MRSA/MRSA rate was significantly associated to PD (p < 0.005).

Conclusion: CC398-MSSA is an emerging clade in invasive infections in Spanish hospitals. CC398-MRSA (mostly t011) and CC398-MSSA (mostly t571 and t1451) show important differences, possibly suggesting divergent steps in host-adaptation evolutionary processes. While CC398-MRSA is livestock-associated (lacking IEC-system), CC398-MSSA seems to be mostly livestock-independent, carrying human-adaptation markers..

Idioma: Inglés

DOI: 10.3389/fmicb.2021.623108

Año: 2021

Publicado en: Frontiers in Microbiology 12 (2021), 623108 [10 pp.]
ISSN: 1664-302X

Factor impacto JCR: 6.064 (2021)

Categ. JCR: MICROBIOLOGY rank: 34 / 138 = 0.246 (2021) - Q1 - T1

Factor impacto CITESCORE: 8.2 - Immunology and Microbiology (Q1) - Medicine (Q1)

Factor impacto SCIMAGO: 1.314 - Microbiology (medical) (Q1) - Microbiology (Q1)

Financiación: info:eu-repo/grantAgreement/ES/MINECO/SAF2016-76571-R

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Microbiología (Dpto. Microb.Ped.Radio.Sal.Pú.)