Effect of glycerol, n, n-dimethylformamide and n-methyl-2-pyrrolidone on rabbit sperm stored at 4 °C and 16 °C

Domingo, P. ; Olaciregui, M. ; González, N. (Universidad de Zaragoza) ; De Blas, I. (Universidad de Zaragoza) ; Gil, L. (Universidad de Zaragoza)

Resumen: Artificial insemination with cooled semen is the most common practice in rabbit farms and any improvement on it helps to increase the efficiency and productivity of rabbit meat farms. Therefore, the aim of this study was to assess whether different cryoprotectant agents (CPA) as glycerol, N, N-Dimethylformamide (DMF) and N-Methyl--2-Pyrrolidone (NMP) can improve cooled rabbit sperm quality stored at 4C and 16C. Sperm samples were diluted with INRA 96® (Extender A), INRA 96® with 6% glycerol (Extender B) or 6% DMF (Extender C) or 6% NMP (Extender D) respectively and stored at 4C and 16C. Samples were then analysed at 4, 24, 48 and 72 hours after refrigeration by integrated sperm analysis system (ISAS), eosin-nigrosin stain (vitality), hypo-osmotic swelling test (HOS test) and acrosome integrity test. Extender C showed higher percentage of motility, vitality and HOS test than extender B and D (p<0.05). Whereas sperm quality decreased over time (p<0.05), data showed that the addition of DMF kept the motility and sperm plasma membrane integrity after 24 hours of storage better than other diluents. These results suggest that the addition of DMF to INRA 96® exerts a protective effect on the membrane of spermatozoa improving seminal quality.

Idioma: Inglés

DOI: 10.21451/1984-3143-AR2018-0100

Año: 2019

Publicado en: Animal Reproduction 16, 4 (2019), 887-894
ISSN: 1806-9614

Factor impacto JCR: 0.916 (2019)

Categ. JCR: AGRICULTURE, DAIRY & ANIMAL SCIENCE rank: 41 / 63 = 0.651 (2019) - Q3 - T2

Factor impacto SCIMAGO: 0.355 - Veterinary (miscellaneous) (Q2) - Animal Science and Zoology (Q3)

Financiación: info:eu-repo/grantAgreement/ES/DGA-FSE/Aragon Research Groups

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)