000099350 001__ 99350
000099350 005__ 20230914083325.0
000099350 0247_ $$2doi$$a10.21451/1984-3143-AR2018-0100
000099350 0248_ $$2sideral$$a122886
000099350 037__ $$aART-2019-122886
000099350 041__ $$aeng
000099350 100__ $$aDomingo, P.
000099350 245__ $$aEffect of glycerol, n, n-dimethylformamide and n-methyl-2-pyrrolidone on rabbit sperm stored at 4 °C and 16 °C
000099350 260__ $$c2019
000099350 5060_ $$aAccess copy available to the general public$$fUnrestricted
000099350 5203_ $$aArtificial insemination with cooled semen is the most common practice in rabbit farms and any improvement on it helps to increase the efficiency and productivity of rabbit meat farms. Therefore, the aim of this study was to assess whether different cryoprotectant agents (CPA) as glycerol, N, N-Dimethylformamide (DMF) and N-Methyl--2-Pyrrolidone (NMP) can improve cooled rabbit sperm quality stored at 4C and 16C. Sperm samples were diluted with INRA 96® (Extender A), INRA 96® with 6% glycerol (Extender B) or 6% DMF (Extender C) or 6% NMP (Extender D) respectively and stored at 4C and 16C. Samples were then analysed at 4, 24, 48 and 72 hours after refrigeration by integrated sperm analysis system (ISAS), eosin-nigrosin stain (vitality), hypo-osmotic swelling test (HOS test) and acrosome integrity test. Extender C showed higher percentage of motility, vitality and HOS test than extender B and D (p<0.05). Whereas sperm quality decreased over time (p<0.05), data showed that the addition of DMF kept the motility and sperm plasma membrane integrity after 24 hours of storage better than other diluents. These results suggest that the addition of DMF to INRA 96® exerts a protective effect on the membrane of spermatozoa improving seminal quality.
000099350 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FSE/Aragon Research Groups
000099350 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000099350 590__ $$a0.916$$b2019
000099350 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b41 / 63 = 0.651$$c2019$$dQ3$$eT2
000099350 592__ $$a0.355$$b2019
000099350 593__ $$aVeterinary (miscellaneous)$$c2019$$dQ2
000099350 593__ $$aAnimal Science and Zoology$$c2019$$dQ3
000099350 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000099350 700__ $$0(orcid)0000-0001-6479-3933$$aOlaciregui, M.
000099350 700__ $$0(orcid)0000-0002-1611-6337$$aGonzález, N.$$uUniversidad de Zaragoza
000099350 700__ $$0(orcid)0000-0002-1204-4356$$aDe Blas, I.$$uUniversidad de Zaragoza
000099350 700__ $$0(orcid)0000-0002-0797-1841$$aGil, L.$$uUniversidad de Zaragoza
000099350 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000099350 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000099350 773__ $$g16, 4 (2019), 887-894$$pAnim. reprod.$$tAnimal Reproduction$$x1806-9614
000099350 8564_ $$s726706$$uhttps://zaguan.unizar.es/record/99350/files/texto_completo.pdf$$yVersión publicada
000099350 8564_ $$s3155782$$uhttps://zaguan.unizar.es/record/99350/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000099350 909CO $$ooai:zaguan.unizar.es:99350$$particulos$$pdriver
000099350 951__ $$a2023-09-13-11:08:28
000099350 980__ $$aARTICLE