Effect of scrapie prion infection in ovine bone marrow-derived mesenchymal stem cells and ovine mesenchymal stem cell-derived neurons

García-Mendívil, Laura (Universidad de Zaragoza) ; Mediano, Diego R. ; Hernaiz, Adelaida (Universidad de Zaragoza) ; Sanz-Rubio, David ; Vázquez, Francisco J. (Universidad de Zaragoza) ; Marín, Belén (Universidad de Zaragoza) ; López-Pérez, Óscar ; Otero, Alicia (Universidad de Zaragoza) ; Badiola, Juan J. (Universidad de Zaragoza) ; Zaragoza, Pilar (Universidad de Zaragoza) ; Ordovás, Laura ; Bolea, Rosa (Universidad de Zaragoza) ; Martín-Burriel, Inmaculada (Universidad de Zaragoza)

Resumen: Scrapie is a prion disease affecting sheep and goats and it is considered a prototype of transmissible spongiform encephalopathies (TSEs). Mesenchymal stem cells (MSCs) have been proposed as candidates for developing in vitro models of prion diseases. Murine MSCs are able to propagate prions after previous mouse-adaptation of prion strains and, although ovine MSCs express the cellular prion protein (PrPC), their susceptibility to prion infection has never been investigated. Here, we analyze the potential of ovine bone marrow-derived MSCs (oBM-MSCs), in growth and neurogenic conditions, to be infected by natural scrapie and propagate prion particles (PrPSc) in vitro, as well as the effect of this infection on cell viability and proliferation. Cultures were kept for 48–72 h in contact with homogenates of central nervous system (CNS) samples from scrapie or control sheep. In growth conditions, oBM-MSCs initially maintained detectable levels of PrPSc post-inoculation, as determined by Western blotting and ELISA. However, the PrPSc signal weakened and was lost over time. oBM-MSCs infected with scrapie displayed lower cell doubling and higher doubling times than those infected with control inocula. On the other hand, in neurogenic conditions, oBM-MSCs not only maintained detectable levels of PrPSc post-inoculation, as determined by ELISA, but this PrPSc signal also increased progressively over time. Finally, inoculation with CNS extracts seems to induce the proliferation of oBM-MSCs in both growth and neurogenic conditions. Our results suggest that oBM-MSCs respond to prion infection by decreasing their proliferation capacity and thus might not be permissive to prion replication, whereas ovine MSC-derived neuron-like cells seem to maintain and replicate PrPSc.

Idioma: Inglés

DOI: 10.3390/ani11041137

Año: 2021

Publicado en: Animals 11, 4 (2021), 1137 [13 pp.]
ISSN: 2076-2615

Factor impacto JCR: 3.231 (2021)

Categ. JCR: VETERINARY SCIENCES rank: 16 / 145 = 0.11 (2021) - Q1 - T1
Categ. JCR: AGRICULTURE, DAIRY & ANIMAL SCIENCE rank: 13 / 62 = 0.21 (2021) - Q1 - T1

Factor impacto CITESCORE: 2.7 - Veterinary (Q2) - Agricultural and Biological Sciences (Q2)

Factor impacto SCIMAGO: 0.61 - Veterinary (miscellaneous) (Q1) - Animal Science and Zoology (Q1)

Financiación: info:eu-repo/grantAgreement/EUR/INTERREG-POCTEFA/EFA-148-16 REDPRION
Financiación: info:eu-repo/grantAgreement/ES/MICINN/RTI2018-098711-B-I00

Tipo y forma: Article (Published version)

Área (Departamento): Área Genética (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)
Área (Departamento): Área Teoría Señal y Comunicac. (Dpto. Ingeniería Electrón.Com.)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)