In vivo potential of recombinant granulysin against human melanoma

Al-Wasaby, S. ; Guerrero-Ochoa, P. (Universidad de Zaragoza) ; Ibáñez-Pérez, R. (Universidad de Zaragoza) ; Soler, R. (Universidad de Zaragoza) ; Conde, B. (Universidad de Zaragoza) ; Martínez-Lostao, L. (Universidad de Zaragoza) ; Anel, A. (Universidad de Zaragoza)

Resumen: 9-kDa granulysin is a protein expressed into the granules of human cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. It has been shown to exert cytolysis on microbes and tumors. We showed previously that 9-kDa granulysin exerted cell death by apoptosis in vitro on hematological tumor cell lines and also on cells from B-cell chronic lymphocytic leukemia (B-CLL) patients. In addition, we have shown the anti-tumor efficiency of granulysin as a single agent in two in vivo models of human tumor development in athymic mice, the MDA-MB-231 mammary adenocarcinoma and the NCI-H929 multiple myeloma, without signs of overt secondary effects by itself. In this work, we have tested recombinant 9-kDa granulysin in an in vivo and especially aggressive model of melanoma development, xenografted UACC62 cells in athymic mice. Recombinant granulysin was administered once UACC62-derived tumors were detectable and it substantially retarded the in vivo development of this aggressive tumor. We could also detect apoptosis induction and increased NK cell infiltration inside granulysin-treated tumor tissues. These observations are especially interesting given the possibility of treating melanoma by intra-tumor injection.

Idioma: Inglés

DOI: 10.1016/j.ctarc.2021.100355

Año: 2021

Publicado en: Cancer Treatment and Research Communications 27 (2021), 100355 [7 pp.]
ISSN: 2468-2942

Factor impacto CITESCORE: 1.5 - Medicine (Q3) - Biochemistry, Genetics and Molecular Biology (Q4)

Factor impacto SCIMAGO: 0.52 - Oncology (Q3) - Cancer Research (Q3)

Financiación: info:eu-repo/grantAgreement/ES/DGA/B31-17R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/SAF2016-76338-R

Tipo y forma: Article (Published version)

Área (Departamento): Area Histología (Dpto. Anatom.Histolog.Humanas)
Área (Departamento): Área Inmunología (Dpto. Microb.Ped.Radio.Sal.Pú.)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Biología Celular (Dpto. Bioq.Biolog.Mol. Celular)