Contributions on lindane degradation by Microcystis aeruginosa PCC 7806

Sarasa-Buisán, C. (Universidad de Zaragoza) ; Guío, J. (Universidad de Zaragoza) ; Castro, C. ; Bes, M. T. (Universidad de Zaragoza) ; Fillat, M. F. (Universidad de Zaragoza) ; Peleato, M. L. (Universidad de Zaragoza) ; Sevilla, E. (Universidad de Zaragoza)

Resumen: Cyanobacteria are able to tolerate, and even metabolize, moderate doses of organochlorine pesticides, such as lindane (¿-hexachlorocyclohexane), one of the most persistent and widely used in recent decades. Previous work showed that Microcystis aeruginosa PCC 7806 degrades lindane and that, in the presence of the pesticide, microcystin synthesis is enhanced. In this work, using in silico approaches, we have identified in M. aeruginosa putative homologues of the lin genes, involved in lindane degradation in Sphingobium japonicum UT26S. Real-time RT-PCR assays showed that the putative linC gene was induced in the presence of 7 mg/L of lindane. Additionally, prxA, encoding a peroxiredoxin, and involved in oxidative stress response, was also induced when lindane was present. Taking into account these results, M. aeruginosa PCC 7806 may degrade lindane through a metabolic pathway involving a putative 2, 5-dichloro-2, 5-cyclohexadiene-1, 4-diol dehydrogenase encoded by a linC homologue. However, the low similarity of the other potential lin homologues suggest the existence of an alternative pathway different to that of heterotrophic microorganisms such as S. japonicum.

Idioma: Inglés

DOI: 10.3390/w14081219

Año: 2022

Publicado en: Water (Switzerland) 14, 8 (2022), 1219 [8 pp.]
ISSN: 2073-4441

Factor impacto JCR: 3.4 (2022)

Categ. JCR: WATER RESOURCES rank: 38 / 103 = 0.369 (2022) - Q2 - T2
Categ. JCR: ENVIRONMENTAL SCIENCES rank: 135 / 275 = 0.491 (2022) - Q2 - T2

Factor impacto CITESCORE: 5.5 - Agricultural and Biological Sciences (Q1) - Social Sciences (Q1) - Environmental Science (Q2) - Biochemistry, Genetics and Molecular Biology (Q2)

Factor impacto SCIMAGO: 0.723 - Geography, Planning and Development (Q1) - Aquatic Science (Q1) - Water Science and Technology (Q2) - Biochemistry (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E35-17R
Financiación: info:eu-repo/grantAgreement/ES/MCIU/PID2019-104889GB-I00
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BFU2016-77671-P

Tipo y forma: Article (Published version)

Área (Departamento): Área Fisiología Vegetal (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)