Neurogranin and Neurofilament Light Chain as Preclinical Biomarkers in Scrapie

Betancor, M. (Universidad de Zaragoza) ; Pérez-Lázaro, S. (Universidad de Zaragoza) ; Otero, A. (Universidad de Zaragoza) ; Marín, B. (Universidad de Zaragoza) ; Martín-Burriel , I. (Universidad de Zaragoza) ; Blennow, K. ; Badiola, J.J. (Universidad de Zaragoza) ; Zetterberg, H. ; Bolea, R. (Universidad de Zaragoza)

Resumen: Prion diseases are diagnosed in the symptomatic stage, when the neuronal damage is spread throughout the central nervous system (CNS). The assessment of biological features that allow the detection of asymptomatic cases is needed, and, in this context, scrapie, where pre-symptomatic infected animals can be detected through rectal biopsy, becomes a good study model. Neurogranin (Ng) and neurofilament light chain (NfL) are proteins that reflect synaptic and axonal damage and have been studied as cerebrospinal fluid (CSF) biomarkers in different neurodegenerative disorders. In this study, we evaluated Ng and NfL both at the protein and transcript levels in the CNS of preclinical and clinical scrapie-affected sheep compared with healthy controls and assessed their levels in ovine CSF. The correlation between these proteins and the main neuropathological events in prion diseases, PrPSc deposition and spongiosis, was also assessed. The results show a decrease in Ng and NfL at the protein and gene expression levels as the disease progresses, and significant changes between the control and preclinical animals. On the contrary, the CSF levels of NfL increased throughout the progression of the disease. Negative correlations between neuropathological markers of prion disease and the concentration of the studied proteins were also found. Although further research is needed, these results suggest that Ng and NfL could act as biomarkers for neurodegeneration onset and intensity in preclinical cases of scrapie.

Idioma: Inglés

DOI: 10.3390/ijms23137182

Año: 2022

Publicado en: International Journal of Molecular Sciences 23, 13 (2022), 7182 [16 pp.]
ISSN: 1661-6596

Factor impacto JCR: 5.6 (2022)

Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 66 / 285 = 0.232 (2022) - Q1 - T1
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 52 / 178 = 0.292 (2022) - Q2 - T1

Factor impacto CITESCORE: 7.8 - Biochemistry, Genetics and Molecular Biology (Q1) - Computer Science (Q1) - Chemistry (Q1) - Chemical Engineering (Q1) - Medicine (Q1)

Factor impacto SCIMAGO: 1.154 - Medicine (miscellaneous) (Q1) - Physical and Theoretical Chemistry (Q1) - Computer Science Applications (Q1) - Inorganic Chemistry (Q1) - Spectroscopy (Q1) - Organic Chemistry (Q1) - Molecular Biology (Q2) - Catalysis (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA-FSE/IIU-2023-2017
Financiación: info:eu-repo/grantAgreement/EC/H2020/860197/EU/Multi-omics Interdisciplinary Research Integration to Address DEmentia diagnosis/MIRIADE
Financiación: info:eu-repo/grantAgreement/EUR/INTERREG-POCTEFA/EFA148-16
Financiación: info:eu-repo/grantAgreement/ES/MICINN/RTI2018-098711-B-I00

Tipo y forma: Article (Published version)

Área (Departamento): Área Genética (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)