Stromal cells of giant cell tumor of bone show primary cilia in giant cell tumor of bone

Castiella, T. ; Iruzubieta, P. ; Monleón, E. (Universidad de Zaragoza) ; Cardiel, M. J. (Universidad de Zaragoza) ; Gómez-Vallejo, J. (Universidad de Zaragoza) ; Monzón, M. (Universidad de Zaragoza) ; Junquera, M. C. (Universidad de Zaragoza)

Resumen: Giant cell tumor of bone (GCTB) is a locally aggressive primary bone neoplasm com-posed by tumoral stromal cells (SCs) and a reactive component that consists ofmonocytic/histiocytic cells that give rise by fusion to osteoclast-like multinucleatedcells. Recently, specific Histone 3.3 mutations have been demonstrated in SCs ofGCTB. Many of the pathways related to bone proliferation and regulation depend onthe primary cilium, a microtubule-based organelle that protrudes outside the cell andacts as a sensorial antenna. In the present work, we aimed to study the presence androle of primary cilia in GCTB. Ultrastructural, immunohistochemical, and immunofluo-rescence studies were performed in order to demonstrate, for the first time, that theprimary cilium is located in spindle-shaped SCs of GCTB. Moreover, we showedHedgehog (Hh) signaling pathway activation in these cells. Hence, primary cilia mayplay a relevant role in GCTB tumorogenesis through Hh signaling activation in SCs.

Idioma: Inglés

DOI: 10.1002/jemt.23976

Año: 2022

Publicado en: MICROSCOPY RESEARCH AND TECHNIQUE 85, 3 (2022), 1065-1074
ISSN: 1059-910X

Factor impacto JCR: 2.5 (2022)

Categ. JCR: ANATOMY & MORPHOLOGY rank: 5 / 20 = 0.25 (2022) - Q1 - T1
Categ. JCR: MICROSCOPY rank: 2 / 8 = 0.25 (2022) - Q1 - T1
Categ. JCR: BIOLOGY rank: 47 / 92 = 0.511 (2022) - Q3 - T2

Factor impacto CITESCORE: 5.2 - Physics and Astronomy (Q2) - Health Professions (Q1) - Medicine (Q2)

Factor impacto SCIMAGO: 0.552 - Medical Laboratory Technology (Q1) - Instrumentation (Q2) - Anatomy (Q2) - Histology (Q2)

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Area Histología (Dpto. Anatom.Histolog.Humanas)
Área (Departamento): Área Traumatología y Ortopedia (Dpto. Cirugía)
Área (Departamento): Área Anatomía Patológica (Dpto. Cirugía)