Pharmacokinetic control on the release of antimicrobial drugs from pH-responsive electrospun wound dressings

Miranda-Calderon, L. ; Yus Argón, C. (Universidad de Zaragoza) ; Landa Baila, G. (Universidad de Zaragoza) ; Mendoza, G. ; Arruebo Gordo, M. (Universidad de Zaragoza) ; Irusta Alderete, S. (Universidad de Zaragoza)

Resumen: The acidic pH of healthy skin changes during wound healing due to the exposure of the inner dermal and subcutaneous tissue and due to the potential colonization of pathogenic bacteria. In chronic non-healing wounds, the pH values vary in a wide pH range but the appearance of an alkaline shift is common. After a wound is incurred, neutral pH in the wound bed is characteristic of the activation of the cascade of regenerative and remodeling processes. In order to adjust drug release to the specific pH of the wound, herein, drug-loaded wound dressings having pH-responsiveness containing antiseptics and antibiotics and exerting different release kinetics in order to have a perfect match between the drug release kinetics, and the pH conditions of each wound type, were developed. We have fabricated drug-loaded electrospun nanofibers loaded with the antiseptic chlorhexidine, with the broad-spectrum antibiotic rifampicin, and with the antimicrobial of natural origin thymol, using the pH-dependent methacrylic acid copolymer Eudragit® L100-55, which dissolves at pH > 5.5; those drugs were loaded within Eudragit® S100, which dissolves at pH > 7 and, finally, within the methacrylic ester copolymer Eudragit® RS100 which is pH independent and slowly erodes and releases its contained cargo. The antibacterial action of those advanced wound dressings has been evaluated against methicillin-sensitive S. aureus Newman strain expressing the coral green fluorescent protein (cGFP), as a model of a Gram-positive bacteria, and against E. coli S17 strain as a model of a Gram-negative bacteria. It was demonstrated that those combinational products integrate in one device the required characteristics for a wound dressing with the therapeutic action of a contained active principle and can be selected depending on the wound acidic or alkaline status for its appropriated management. © 2022 The Author(s)

Idioma: Inglés

DOI: 10.1016/j.ijpharm.2022.122003

Año: 2022

Publicado en: International Journal of Pharmaceutics 624 (2022), 122003 [11 pp]
ISSN: 0378-5173

Factor impacto JCR: 5.8 (2022)

Categ. JCR: PHARMACOLOGY & PHARMACY rank: 39 / 278 = 0.14 (2022) - Q1 - T1

Factor impacto CITESCORE: 10.5 - Pharmacology, Toxicology and Pharmaceutics (Q1)

Factor impacto SCIMAGO: 0.906 - Pharmaceutical Science (Q1)

Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2020-113987RB-I00

Tipo y forma: Article (Published version)

Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)
Área (Departamento): Área Tecnologi. Medio Ambiente (Dpto. Ing.Quím.Tecnol.Med.Amb.)