DNA-Liposome Hybrid Carriers for Triggered Cargo Release

Baumann, K. N. ; Schröder, T. ; Ciryam, P. S. ; Morzy, D. ; Tinnefeld, P. ; Knowles, T. P. J. ; Hernández-Ainsa, S. (Universidad de Zaragoza)

Resumen: The design of simple and versatile synthetic routes to accomplish triggered-release properties in carriers is of particular interest for drug delivery purposes. In this context, the programmability and adaptability of DNA nanoarchitectures in combination with liposomes have great potential to render biocompatible hybrid carriers for triggered cargo release. We present an approach to form a DNA mesh on large unilamellar liposomes incorporating a stimuli-responsive DNA building block. Upon incubation with a single-stranded DNA trigger sequence, a hairpin closes, and the DNA building block is allowed to self-contract. We demonstrate the actuation of this building block by single-molecule Förster resonance energy transfer (FRET), fluorescence recovery after photobleaching, and fluorescence quenching measurements. By triggering this process, we demonstrate the elevated release of the dye calcein from the DNA-liposome hybrid carriers. Interestingly, the incubation of the doxorubicin-laden active hybrid carrier with HEK293T cells suggests increased cytotoxicity relative to a control carrier without the triggered-release mechanism. In the future, the trigger could be provided by peritumoral nucleic acid sequences and lead to site-selective release of encapsulated chemotherapeutics. © 2022 American Chemical Society. All rights reserved.

Idioma: Inglés

DOI: 10.1021/acsabm.2c00225

Año: 2022

Publicado en: ACS Applied Bio Materials 5, 8 (2022), 3713-3721
ISSN: 2576-6422

Factor impacto CITESCORE: 7.9 - Engineering (Q1) - Chemistry (Q1) - Medicine (Q1) - Materials Science (Q1)

Factor impacto SCIMAGO: 0.855 - Chemistry (miscellaneous) (Q1) - Biomedical Engineering (Q2) - Biochemistry (medical) (Q2) - Biomaterials (Q2)

Financiación: info:eu-repo/grantAgreement/EC/FP7/337969/EU/Determining Physical Properties of Heterogeneous Protein Complexes in Small Volumes/PHYSPROT

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Química Orgánica (Dpto. Química Orgánica)