Nuclear DNA fragmentation in boar spermatozoa: measurement methods and reproductive performance implications

Ausejo, R. (Universidad de Zaragoza) ; Martínez, J. M. (Universidad de Zaragoza) ; Mendoza, N. ; Bolarin, A. ; Tejedor, M. T. (Universidad de Zaragoza) ; Falceto, M. V. (Universidad de Zaragoza)

Resumen: The aim of this research was to compare the different techniques to measure sperm nuclear DNA fragmentation (sDF) and to check its relations to boar reproductive value, classical spermiogram parameters, and reproductive results of the doses in sows. Sperm chromatin stability assay (SCSA), terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, and sperm chromatin dispersion test (SCD, Halomax®) results were compared, finding a statistically significant correlation only between SCSA and TUNEL results. The fertility direct boar effect (DBE) index, calculated from the whole productive life of the boar, was not correlated (p > 0.05) with sDF (measured by any technique). Total or progressive sperm motility was not correlated with sDF, while it found a positive correlation between TUNEL measure and abnormal acrosomes (%) and between SCD measure and total sperm morphological abnormalities (%). No significant correlations were obtained between fertility or prolificacy results and sDF results with the different techniques. However, in the case of total born and SCSA measure, the correlation was close to significance (r partial = -0.095; p = 0.066), appointing to a tendency; as SCSA increases, the number of total piglets born decreases. In conclusion, although the different techniques for the sDF seem not to target exactly the same DNA events and the relationship between their values and the reproductive results and the classical spermiogram results is still to be elucidated, the studied sDF techniques may offer extra information that could be useful for the management of AI studs. Copyright © 2022 Ausejo, Martínez, Mendoza, Bolarin, Tejedor and Falceto.

Idioma: Inglés

DOI: 10.3389/fvets.2022.929858

Año: 2022

Publicado en: Frontiers in Veterinary Science 9 (2022), 929858 [11 pp]
ISSN: 2297-1769

Factor impacto JCR: 3.2 (2022)

Categ. JCR: VETERINARY SCIENCES rank: 12 / 144 = 0.083 (2022) - Q1 - T1

Factor impacto CITESCORE: 3.8 - Veterinary (Q1)

Factor impacto SCIMAGO: 0.737 - Veterinary (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/UZ/III convocatoria de ayudas para el Desarrollo del programa de doctorados industriales-empresariales

Tipo y forma: Article (Published version)

Área (Departamento): Área Genética (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)
Área (Departamento): Área Tecnología de Alimentos (Dpto. Produc.Animal Cienc.Ali.)