Globotriaosylceramide-related biomarkers of fabry disease identified in plasma by high-performance thin-layer chromatography - densitometry- mass spectrometry

Jarne, C. ; Membrado, L. ; Savirón, M. ; Vela, J. (Universidad de Zaragoza) ; Orduna, J. ; Garriga, R. (Universidad de Zaragoza) ; Galbán, J. (Universidad de Zaragoza) ; Cebolla, V.L.

Resumen: Identification of 19 molecular species of globotriaosylceramides (Gb3) in extracts from a Fabry''s plasma patient and a healthy control was performed by High-Performance Thin-Layer Chromatography (HPTLC)-densitometry and online coupling to Mass Spectrometry (MS). Separation was carried out on LiChrospher plates using Automated Multiple Development (AMD). Densitometry was performed on twin plates by combining detection in the visible at 550 nm, through previous on-plate orcinol derivatization, and by Ultraviolet 190 nm, using a non-impregnated plate. The latter was directly coupled to an ion-trap mass spectrometer through an automated elution-based interface. Gb3 molecular species, which were identified by HPTLC- Electrospray Mass Spectrometry (+)-MS and confirmed by MS/MS or HPTLC-Atmospheric Pressure Chemical Ionization Mass Spectrometry (+)-MS, are: five isoforms of saturated Gb3; seven isoforms of methylated Gb3; and seven species with two additional double bonds. Twelve of these species were previously reported as biomarkers of Fabry''s lysosomal disorder using a Liquid Chromatography-MS-based method, and the other seven are structurally similar, closely related to them. Saturated Gb3 isoforms migrated on LiChrospher plate in one of the separated peaks corresponding to the migration zone of ceramide trihexosides standard. Instead, methylated and unsaturated Gb3 species co-migrated with sphingomyelin species. Ion intensity ESI-MS profiles show that saturated Gb3 species in Fabry''s plasma were in higher concentration than in control sample. Before applying the Thin-Layer Chromatography (TLC)-MS interface on HPTLC separated peaks, its positioning precision was first studied using ceramide tri-hexosides as model compound. This provided information on Gb3 peak broadening and splitting during its migration.

Idioma: Inglés

DOI: 10.1016/j.chroma.2021.461895

Año: 2021

Publicado en: Journal of Chromatography A 1638 (2021), 461895 [11 pp.]
ISSN: 0021-9673

Factor impacto JCR: 4.601 (2021)

Categ. JCR: CHEMISTRY, ANALYTICAL rank: 20 / 87 = 0.23 (2021) - Q1 - T1
Categ. JCR: BIOCHEMICAL RESEARCH METHODS rank: 23 / 79 = 0.291 (2021) - Q2 - T1

Factor impacto CITESCORE: 7.3 - Medicine (Q1) - Biochemistry, Genetics and Molecular Biology (Q1)

Factor impacto SCIMAGO: 0.813 - Analytical Chemistry (Q1) - Organic Chemistry (Q1) - Medicine (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E25-17R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/CTQ2016-76846-R

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Química Analítica (Dpto. Química Analítica)
Área (Departamento): Área Química Física (Dpto. Química Física)