Local temperature increments and induced cell death in intracellular magnetic hyperthermia

Gu, Y. ; Piñol, R. ; Moreno-Loshuertos, R. (Universidad de Zaragoza) ; Brites, C. D. S. ; Zeler, J. ; Martínez, A. (Universidad de Zaragoza) ; Maurin-Pasturel, G. ; Fernández-Silva, P. (Universidad de Zaragoza) ; Marco-Brualla, J. (Universidad de Zaragoza) ; Téllez, P. (Universidad de Zaragoza) ; Cases, R. (Universidad de Zaragoza) ; Navarro Belsué, R. ; Bonvin, D. ; Carlos, L. D. ; Millán, Á.

Resumen: The generation of temperature gradients on nanoparticles heated externally by a magnetic field is crucially important in magnetic hyperthermia therapy. But the intrinsic low heating power of magnetic nanoparticles, at the conditions allowed for human use, is a limitation that restricts the general implementation of the technique. A promising alternative is local intracellular hyperthermia, whereby cell death (by apoptosis, necroptosis, or other mechanisms) is attained by small amounts of heat generated at thermosensitive intracellular sites. However, the few experiments conducted on the temperature determination of magnetic nanoparticles have found temperature increments that are much higher than the theoretical predictions, thus supporting the local hyperthermia hypothesis. Reliable intracellular temperature measurements are needed to get an accurate picture and resolve the discrepancy. In this paper, we report the real-time variation of the local temperature on γ-Fe2O3 magnetic nanoheaters using a Sm3+/Eu3+ ratiometric luminescent thermometer located on its surface during exposure to an external alternating magnetic field. We measure maximum temperature increments of 8 °C on the surface of the nanoheaters without any appreciable temperature increase on the cell membrane. Even with magnetic fields whose frequency and intensity are still well within health safety limits, these local temperature increments are sufficient to produce a small but noticeable cell death, which is enhanced considerably as the magnetic field intensity is increased to the maximum level tolerated for human use, consequently demonstrating the feasibility of local hyperthermia.

Idioma: Inglés

DOI: 10.1021/acsnano.3c00388

Año: 2023

Publicado en: ACS NANO 17, 7 (2023), 6822-6832
ISSN: 1936-0851

Factor impacto JCR: 15.8 (2023)

Categ. JCR: CHEMISTRY, PHYSICAL rank: 14 / 178 = 0.079 (2023) - Q1 - T1
Categ. JCR: NANOSCIENCE & NANOTECHNOLOGY rank: 11 / 140 = 0.079 (2023) - Q1 - T1
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 14 / 230 = 0.061 (2023) - Q1 - T1
Categ. JCR: MATERIALS SCIENCE, MULTIDISCIPLINARY rank: 27 / 438 = 0.062 (2023) - Q1 - T1

Factor impacto SCIMAGO: 4.593 - Engineering (miscellaneous) (Q1) - Physics and Astronomy (miscellaneous) (Q1) - Nanoscience and Nanotechnology (Q1) - Materials Science (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E11-17R
Financiación: info:eu-repo/grantAgreement/ES/DGA/LMP220_21
Financiación: info:eu-repo/grantAgreement/EC/H2020/801305/EU/Nanoparticles-based 2D thermal bioimaging technologies/NanoTBTech
Financiación: info:eu-repo/grantAgreement/EC/H2020/829162/EU/Redesigning biocatalysis: Thermal-tuning of one-pot multienzymatic cascades by nanoactuation/HOTZYMES
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PGC2018-095795-B-I00
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2021-124354NB-I00

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Tecnología Electrónica (Dpto. Ingeniería Electrón.Com.)
Área (Departamento): División: Serv. Transversales (Serv.Gral. Apoyo Investigación)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Física Materia Condensada (Dpto. Física Materia Condensa.)