X-ray Photoelectron Spectroscopy (XPS) analysis of nitrogen environment in small extracellular vesicle membranes: a potential novel technique with application for cancer screening

Sancho-Albero, María (Universidad de Zaragoza) ; Martín-Pardillos, Ana (Universidad de Zaragoza) ; Irusta, Silvia (Universidad de Zaragoza) ; Sebastián, Víctor (Universidad de Zaragoza) ; Cebolla, Vicente Luis ; Pazo-Cid, Roberto ; Martín-Duque, Pilar (Universidad de Zaragoza) ; Santamaría, Jesús (Universidad de Zaragoza)

Resumen: Small extracellular vesicle (EV) membranes display characteristic protein-lipidic composition features that are related to their cell of origin, providing valuable clues regarding their parental cell composition and real-time state. This could be especially interesting in the case of cancer cell-derived EVs, as their membranes could serve as valuable tools in liquid biopsy applications and to detect changes in the tumor malignancy. X-Ray Photoelectron Spectroscopy (XPS) is a powerful surface analysis technique able to detect every chemical element present, being also sensitive to their chemical environment. Here we explore the use of XPS as a fast technique to characterize EV membrane composition, with possible application in cancer research. Notably, we have focused on the nitrogen environment as an indicator of the relative abundance of pyridine-type bonding, primary, secondary and tertiary amines. Specifically, we have analyzed how tumoral and healthy cells have different nitrogen chemical environments that can indicate the presence or absence of malignancy. In addition, a collection of human serum samples from cancer patients and healthy donors was also analyzed. The differential XPS analysis of EVs collected from patients confirmed that the patterns of amine evolution could be related to markers of cancer disease, opening the possibility of their use as a non-invasive blood biomarker.

Idioma: Inglés

DOI: 10.3390/cancers15092479

Año: 2023

Publicado en: Cancers 15, 9 (2023), 2479 [18 pp.]
ISSN: 2072-6694

Factor impacto JCR: 4.5 (2023)

Categ. JCR: ONCOLOGY rank: 78 / 322 = 0.242 (2023) - Q1 - T1

Factor impacto CITESCORE: 8.0 - Oncology (Q1) - Cancer Research (Q2)

Factor impacto SCIMAGO: 1.391 - Oncology (Q1) - Cancer Research (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/B054-12
Financiación: info:eu-repo/grantAgreement/ES/DGA-FEDER/Una manera de hacer Europa
Financiación: info:eu-repo/grantAgreement/ES/DGA-FSE/Building Europe from Aragon
Financiación: info:eu-repo/grantAgreement/EC/H2020/742684/EU/Catalytic Dual-Function Devices Against Cancer/CADENCE
Financiación: info:eu-repo/grantAgreement/ES/ISCIII DTS21-00130
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/PI19-01007

Tipo y forma: Article (Published version)

Área (Departamento): Área Estomatología (Dpto. Cirugía)
Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)
Área (Departamento): Área Tecnologi. Medio Ambiente (Dpto. Ing.Quím.Tecnol.Med.Amb.)