Expanding the FurC (PerR) regulon in Anabaena (Nostoc) sp. PCC 7120: Genome-wide identification of novel direct targets uncovers FurC participation in central carbon metabolism regulation

Sarasa-Buisan, Cristina ; Guío, Jorge (Universidad de Zaragoza) ; Peleato, M. Luisa ; Fillat, María F. (Universidad de Zaragoza) ; Sevilla, Emma (Universidad de Zaragoza)

Resumen: FurC (PerR, Peroxide Response Regulator) from Anabaena sp. PCC 7120 (also known as Nostoc sp. PCC 7120) is a master regulator engaged in the modulation of relevant processes including the response to oxidative stress, photosynthesis and nitrogen fixation. Previous differential gene expression analysis of a furC-overexpressing strain (EB2770FurC) allowed the inference of a putative FurC DNA-binding consensus sequence. In the present work, more data concerning the regulon of the FurC protein were obtained through the searching of the putative FurC-box in the whole Anabaena sp. PCC 7120 genome. The total amount of novel FurC-DNA binding sites found in the promoter regions of genes with known function was validated by electrophoretic mobility shift assays (EMSA) identifying 22 new FurC targets. Some of these identified targets display relevant roles in nitrogen fixation (hetR and hgdC) and carbon assimilation processes (cmpR, glgP1 and opcA), suggesting that FurC could be an additional player for the harmonization of carbon and nitrogen metabolisms. Moreover, differential gene expression of a selection of newly identified FurC targets was measured by Real Time RT-PCR in the furC-overexpressing strain (EB2770FurC) comparing to Anabaena sp. PCC 7120 revealing that in most of these cases FurC could act as a transcriptional activator.

Idioma: Inglés

DOI: 10.1371/journal.pone.0289761

Año: 2023

Publicado en: PLoS ONE 18, 8 (2023), e0289761 [19 pp]
ISSN: 1932-6203

Factor impacto JCR: 2.9 (2023)

Categ. JCR: MULTIDISCIPLINARY SCIENCES rank: 32 / 134 = 0.239 (2023) - Q1 - T1

Factor impacto CITESCORE: 6.2 - Multidisciplinary (Q1)

Factor impacto SCIMAGO: 0.839 - Multidisciplinary (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E35-20R
Financiación: info:eu-repo/grantAgreement/ES/MCIU/PID2019-104889GB-I00

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Fisiología Vegetal (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)