Pivotal role of the protein corona in the cell uptake of fluorinated nanoparticles with increased sensitivity for19F-MR imaging
Gatti, Lodovico ; Chirizzi, Cristina ; Rotta, Giulia ; Milesi, Pietro ; Sancho-Albero, María (Universidad de Zaragoza) ; Sebastián, Victor (Universidad de Zaragoza) ; Mondino, Anna ; Santamaría, Jesús (Universidad de Zaragoza) ; Metrangolo, Pierangelo ; Chaabane, Linda ; Baldelli Bombelli, Francesca
Resumen: In vivo cell tracking by non-invasive imaging technologies is needed to accelerate the clinical translation of innovative cell-based therapies. In this regard, 19F-MRI has recently gained increased attention for unbiased localization of labeled cells over time. To push forward the use of 19F-MRI for cell tracking, the development of highly performant 19F-probes is required. PLGA-based NPs containing PERFECTA, a multibranched superfluorinated molecule with an optimal MRI profile thanks to its 36 magnetically equivalent fluorine atoms, are promising 19F-MRI probes. In this work we demonstrate the importance of the surface functionalization of these NPs in relation to their interaction with the biological environment, stressing the pivotal role of the formation of the protein corona (PC) in their cellular labelling efficacy. In particular, our studies showed that the formation of PC NPs strongly promotes the cellular internalization of these NPs in microglia cells. We advocate that the formation of PC NPs in the culture medium can be a key element to be used for the optimization of cell labelling with a considerable increase of the detection sensitivity by 19F-MRI.
Idioma: Inglés
DOI: 10.1039/d3na00229b
Año: 2023
Publicado en: Nanoscale Advances 5, 14 (2023), 3749-3760
ISSN: 2516-0230
Factor impacto JCR: 4.6 (2023)
Categ. JCR: MATERIALS SCIENCE, MULTIDISCIPLINARY rank: 141 / 439 = 0.321 (2023) - Q2 - T1
Categ. JCR: NANOSCIENCE & NANOTECHNOLOGY rank: 60 / 141 = 0.426 (2023) - Q2 - T2
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 69 / 231 = 0.299 (2023) - Q2 - T1
Factor impacto CITESCORE: 8.0 - Atomic and Molecular Physics, and Optics (Q1) - Engineering (all) (Q1) - Chemistry (all) (Q1) - Materials Science (all) (Q1) - Bioengineering (Q2)
Factor impacto SCIMAGO: 0.953 - Atomic and Molecular Physics, and Optics (Q1) - Engineering (miscellaneous) (Q1) - Materials Science (miscellaneous) (Q1) - Chemistry (miscellaneous) (Q1) - Bioengineering (Q2)
Tipo y forma: Article (Published version)
Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes.
Exportado de SIDERAL (2024-11-22-11:58:25)
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Idioma: Inglés
DOI: 10.1039/d3na00229b
Año: 2023
Publicado en: Nanoscale Advances 5, 14 (2023), 3749-3760
ISSN: 2516-0230
Factor impacto JCR: 4.6 (2023)
Categ. JCR: MATERIALS SCIENCE, MULTIDISCIPLINARY rank: 141 / 439 = 0.321 (2023) - Q2 - T1
Categ. JCR: NANOSCIENCE & NANOTECHNOLOGY rank: 60 / 141 = 0.426 (2023) - Q2 - T2
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 69 / 231 = 0.299 (2023) - Q2 - T1
Factor impacto CITESCORE: 8.0 - Atomic and Molecular Physics, and Optics (Q1) - Engineering (all) (Q1) - Chemistry (all) (Q1) - Materials Science (all) (Q1) - Bioengineering (Q2)
Factor impacto SCIMAGO: 0.953 - Atomic and Molecular Physics, and Optics (Q1) - Engineering (miscellaneous) (Q1) - Materials Science (miscellaneous) (Q1) - Chemistry (miscellaneous) (Q1) - Bioengineering (Q2)
Tipo y forma: Article (Published version)
Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. Exportado de SIDERAL (2024-11-22-11:58:25)
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Record created 2023-10-23, last modified 2024-11-25