Coralyne Cation, a Fluorescent Probe for General Detection in Planar Chromatography

Mateos, E. (Universidad de Zaragoza) ; Cebolla,V. L. ; Membrado,L. ; Vela,J. (Universidad de Zaragoza) ; Galvez,E. M. ; Matt,M. ; Cossio,F. P.

Resumen: A large number of analytes, including non-fluorescent ones, can be sensitively detected by fluorescence scanning densitometry using silica gel HPTLC plates impregnated with a solution of coralyne cation. This is carried out by the variation, increase or decrease, that the corresponding analyte induces on native coralyne emission at a given excitation wavelength. A similar phenomenon was previously described for berberine cation, and Reichardt's dye probes. However, the sensitivity of coralyne in HPTLC detection of non-fluorescent, structurally different analytes (e.g., long-chain alkanes, alcohols, alkylbromides, neutral lipids) is superior to that of the above-mentioned probes. In this work, the analytical viability of this phenomenon for HPTLC detection using coralyne as a probe is explored, and fluorescent responses of a number of analytes on the coralyne system are rationalized in the light of a previously proposed model. This establishes that the resulting intensity for a probe in the presence of a given compound can be explained as a balance between radiative (contribution of non-specific interactions) and non-radiative processes (specific interactions), the latter producing fluorescence quenching. Experimental results and proposed model suggest that this phenomenon may be general for practically all kinds of analytes.

Idioma: Inglés

DOI: 10.1016/j.chroma.2007.01.138

Año: 2007

Publicado en: Journal of Chromatography A 1146, 2 (2007), 251-257
ISSN: 0021-9673

Factor impacto JCR: 3.641 (2007)

Categ. JCR: CHEMISTRY, ANALYTICAL rank: 5 / 70 = 0.071 (2007) - Q1 - T1
Categ. JCR: BIOCHEMICAL RESEARCH METHODS rank: 15 / 59 = 0.254 (2007) - Q2 - T1

Financiación: info:eu-repo/grantAgreement/ES/DGA/PM010-2006
Financiación: info:eu-repo/grantAgreement/ES/MEC/CTQ2005-00227-PPQ

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Química Analítica (Dpto. Química Analítica)
Área (Departamento): Proy. investigación HVA (Dpto. Química Analítica)