Proteomics and gene expression analyses of squalene-supplemented mice identify microsomal thioredoxin domain-containing protein 5 changes associated with hepatic steatosis

Ramírez-Torres, A ; Barceló-Batllori, S ; Martínez-Beamonte, R ; Navarro, MA ; Surra, JC (Universidad de Zaragoza) ; Arnal, C (Universidad de Zaragoza) ; Guillén, N (Universidad de Zaragoza) ; Acín, S ; Osada, J (Universidad de Zaragoza)

Resumen: Squalene is an abundant hydrocarbon present in virgin olive oil. Previous studies showed that its administration decreased atherosclerosis and steatosis in male apoE-knock-out mice. To study its effects on microsomal proteins, 1 g/kg/day of squalene was administered to those mice. After 10 weeks, hepatic fat content was assessed and protein extracts of microsomal enriched fractions from control and squalene-treated animals were analyzed by 2D-DIGE. Spots exhibiting significant differences were identified by peptide fingerprinting and MSMS analysis. Squalene administration modified the expression of thirty-one proteins involved in different metabolic functions and increased the levels of those involved in vesicle transport, protein folding and redox status. Only mRNA levels of 9 genes (Arg1, Atp5b, Cat, Hyou1, Nipsnap1, Pcca, Pcx, Pyroxd2, and Txndc5) paralleled these findings. No such mRNA changes were observed in wild-type mice receiving squalene. Thioredoxin domain-containing protein 5 (TXNDC5) protein and mRNA levels were significantly associated with hepatic fat content in apoE-ko mice. These results suggest that squalene action may be executed through a complex regulation of microsomal proteins, both at the mRNA and post-transcriptional levels and the presence of apoE may change the outcome. Txndc5 reflects the anti-steatotic properties of squalene and the sensitivity to lipid accumulation.

Idioma: Inglés

DOI: 10.1016/j.jprot.2012.07.001

Año: 2012

Publicado en: JOURNAL OF PROTEOMICS 77 (2012), 27-39
ISSN: 1874-3919

Factor impacto JCR: 4.088 (2012)

Categ. JCR: BIOCHEMICAL RESEARCH METHODS rank: 15 / 74 = 0.203 (2012) - Q1 - T1

Financiación: info:eu-repo/grantAgreement/ES/CICYT-FEDER/SAF 2010-14958
Financiación: info:eu-repo/grantAgreement/ES/DGA-FSE/B69
Financiación: info:eu-repo/grantAgreement/ES/DGA/PI025-08

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)
Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Area Toxicología (Dpto. Anat.Pat.Med.Leg.For.To.)