In vitro maintenance of drones and development of a new software for sperm quality analysis facilitate the study of honey bee reproductive quality
Divasón, José ; Romero, Ana ; Silvestre, Miguel Á ; Santolaria, Pilar (Universidad de Zaragoza) ; Yániz, Jesús L. (Universidad de Zaragoza)
Resumen: This study aimed to develop a laboratory method that allows the in vitro maintenance of honey bee drones for several days while preserving their reproductive capacity and to create a new open-source software for the automatic analysis of their sperm quality. Three experiments were performed. The first experiment was designed to validate the new open-source software named CASABee for sperm quality assessment specifically designed for the honey bee. The software was able to identify motile and static spermatozoa with high precision. Results showed a high correlation between the results of sperm quality obtained both manually and by the CASABee system (0.95 and 0.96 for sperm motility and concentration, respectively, p < 0.001). In the second and third experiments, the effect of in vitro maintenance of drones without attendant workers for four days on their ejaculatory capacity and sperm quality, respectively, was evaluated. Survival rate was 98.68%, 89.48%, 75.93%, and 60.97% on average on days 1, 2, 3, and 4 after capturing, respectively. A high proportion of the drones (80.37% on average) were able to ejaculate providing semen, and there were no significant differences in the ejaculatory capacity and sperm quality of drones on the different days of in vitro maintenance, except for sperm viability, which decreased slightly on day 4 (71.71% vs 82.8% on day 0, p < 0.05). It was concluded that the new CASABee system and the method for laboratory maintenance of honey bee drones facilitate the study of reproduction in this species.
Idioma: Inglés
DOI: 10.1080/00218839.2023.2231673
Año: 2023
Publicado en: JOURNAL OF APICULTURAL RESEARCH 63, 5 (2023), 1088-1095
ISSN: 0021-8839
Factor impacto JCR: 1.4 (2023)
Categ. JCR: ENTOMOLOGY rank: 46 / 109 = 0.422 (2023) - Q2 - T2
Factor impacto CITESCORE: 4.8 - Insect Science (Q1)
Factor impacto SCIMAGO: 0.449 - Insect Science (Q2)
Financiación: info:eu-repo/grantAgreement/ES/AEI/PID2020-112673RB-I00
Financiación: info:eu-repo/grantAgreement/ES/AEI/PID2020-116641GB-I00
Financiación: info:eu-repo/grantAgreement/ES/DGA-FSE/A07-17R
Tipo y forma: Article (PostPrint)
Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes.
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Idioma: Inglés
DOI: 10.1080/00218839.2023.2231673
Año: 2023
Publicado en: JOURNAL OF APICULTURAL RESEARCH 63, 5 (2023), 1088-1095
ISSN: 0021-8839
Factor impacto JCR: 1.4 (2023)
Categ. JCR: ENTOMOLOGY rank: 46 / 109 = 0.422 (2023) - Q2 - T2
Factor impacto CITESCORE: 4.8 - Insect Science (Q1)
Factor impacto SCIMAGO: 0.449 - Insect Science (Q2)
Financiación: info:eu-repo/grantAgreement/ES/AEI/PID2020-112673RB-I00
Financiación: info:eu-repo/grantAgreement/ES/AEI/PID2020-116641GB-I00
Financiación: info:eu-repo/grantAgreement/ES/DGA-FSE/A07-17R
Tipo y forma: Article (PostPrint)
Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. Exportado de SIDERAL (2024-12-20-12:05:29)
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Record created 2024-01-23, last modified 2024-12-20