Development and validation of a rapid lateral flow test for the detection of fluoroquinolones in meat and blood
Serrano, María Jesús ; Mata, Luis ; Pellicer, Silvia ; Segura-Gil, Isabel ; Razquin, Pedro ; Pagán, Rafael (Universidad de Zaragoza)
Resumen: Fluoroquinolones are antibiotics of considerable relevance in veterinary therapy. If they fail to be properly controlled, they can eventually reach animal-derived food products. As most of the biologically based screening tests used in surveillance plans are not able to detect fluoroquinolones, it would be highly convenient to devise a complementary test designed to detect them specifically. Therefore, a new lateral flow immunoassay test was developed for the ante and post mortem detection of fluoroquinolones in blood and meat samples, respectively, of the main food-producing species. In both matrixes, the test was able to detect most of the European Union-authorized fluoroquinolones at the Maximum Residue Limits set in the European Union for meat; as an instance, the limit of detection was 50 μg/kg for enrofloxacin, 200 μg/kg for flumequine, and 100 μg/kg for marbofloxacin. Hence, this new technique is proposed as an automatized, sensitive, specific, rapid, and robust tool for the management of fluoroquinolone residues in all stages of meat production: no only as a post morten method, in meat samples, but also as an ante mortem method, in blood samples from living animals.
Idioma: Inglés
DOI: 10.1016/j.foodcont.2023.110116
Año: 2023
Publicado en: Food Control 156 (2023), 110116 [8 pp.]
ISSN: 0956-7135
Factor impacto JCR: 5.6 (2023)
Categ. JCR: FOOD SCIENCE & TECHNOLOGY rank: 24 / 173 = 0.139 (2023) - Q1 - T1
Factor impacto CITESCORE: 12.2 - Biotechnology (Q1) - Food Science (Q1)
Factor impacto SCIMAGO: 1.146 - Food Science (Q1) - Biotechnology (Q1)
Tipo y forma: Article (Published version)
Área (Departamento): Área Tecnología de Alimentos (Dpto. Produc.Animal Cienc.Ali.)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes.
Exportado de SIDERAL (2024-07-31-09:52:27)
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Idioma: Inglés
DOI: 10.1016/j.foodcont.2023.110116
Año: 2023
Publicado en: Food Control 156 (2023), 110116 [8 pp.]
ISSN: 0956-7135
Factor impacto JCR: 5.6 (2023)
Categ. JCR: FOOD SCIENCE & TECHNOLOGY rank: 24 / 173 = 0.139 (2023) - Q1 - T1
Factor impacto CITESCORE: 12.2 - Biotechnology (Q1) - Food Science (Q1)
Factor impacto SCIMAGO: 1.146 - Food Science (Q1) - Biotechnology (Q1)
Tipo y forma: Article (Published version)
Área (Departamento): Área Tecnología de Alimentos (Dpto. Produc.Animal Cienc.Ali.)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. Exportado de SIDERAL (2024-07-31-09:52:27)
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Record created 2024-03-11, last modified 2024-07-31