Enhanced Efficiency of Pd(0)-Based Single Chain Polymeric Nanoparticles for <i>in Vitro</i> Prodrug Activation by Modulating the Polymer’s Microstructure

Deng, Linlin ; Sathyan, Anjana ; Adam, Catherine ; Unciti-Broceta, Asier ; Sebastian, Víctor (Universidad de Zaragoza) ; Palmans, Anja R. A.

Resumen: Bioorthogonal catalysis employing transition metal catalysts is a promising strategy for the in situ synthesis of imaging and therapeutic agents in biological environments. The transition metal Pd has been widely used as a bioorthogonal catalyst, but bare Pd poses challenges in water solubility and catalyst stability in cellular environments. In this work, Pd(0) loaded amphiphilic polymeric nanoparticles are applied to shield Pd in the presence of living cells for the in situ generation of a fluorescent dye and anticancer drugs. Pd(0) loaded polymeric nanoparticles prepared by the reduction of the corresponding Pd(II)-polymeric nanoparticles are highly active in the deprotection of pro-rhodamine dye and anticancer prodrugs, giving significant fluorescence enhancement and toxigenic effects, respectively, in HepG2 cells. In addition, we show that the microstructure of the polymeric nanoparticles for scaffolding Pd plays a critical role in tuning the catalytic efficiency, with the use of the ligand triphenylphosphine as a key factor for improving the catalyst stability in biological environments.

Idioma: Inglés

DOI: 10.1021/acs.nanolett.3c04466

Año: 2024

Publicado en: Nano Letters 24, 7 (2024), 2242-2249
ISSN: 1530-6984

Financiación: info:eu-repo/grantAgreement/ES/MICINN/PDC2022-133866-I00
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2021-127847OB-I00

Tipo y forma: Article (Published version)

Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)