Multiparametric in vitro and in vivo analysis of the safety profile of self-assembling peptides

Ramirez-Labrada, Ariel ; Santiago, Llipsy ; Pesini, Cecilia ; Arrieta, Marta ; Arias, Maykel ; Calvo Pérez, Adanays ; Ciulla, Maria Gessica ; Forouharshad, Mahdi ; Pardo, Julian (Universidad de Zaragoza) ; Gálvez, Eva M. ; Gelain, Fabrizio

Resumen: Self-assembling peptides (SAPs) have gained significant attention in biomedicine because of their unique properties and ability to undergo molecular self-assembly driven by non-covalent interactions. By manipulating their composition and structure, SAPs can form well-ordered nanostructures with enhanced selectivity, stability and biocompatibility. SAPs offer advantages such as high chemical and biological diversity and the potential for functionalization. However, studies concerning its potentially toxic effects are very scarce, a limitation that compromises its potential translation to humans. This study investigates the potentially toxic effects of six different SAP formulations composed of natural amino acids designed for nervous tissue engineering and amenable to ready cross-linking boosting their biomechanical properties. All methods were performed in accordance with the relevant guidelines and regulations. A wound-healing assay was performed to evaluate how SAPs modify cell migration. The results in vitro demonstrated that SAPs did not induce genotoxicity neither skin sensitization. In vivo, SAPs were well-tolerated without any signs of acute systemic toxicity. Interestingly, SAPs were found to promote the migration of endothelial, macrophage, fibroblast, and neuronal-like cells in vitro, supporting a high potential for tissue regeneration. These findings contribute to the development and translation of SAP-based biomaterials for biomedical applications.

Idioma: Inglés

DOI: 10.1038/s41598-024-54051-7

Año: 2024

Publicado en: Scientific reports (Nature Publishing Group) 14, 1 (2024), 4395 [19 pp.]
ISSN: 2045-2322

Financiación: info:eu-repo/grantAgreement/ES/AEI/PID2020-113963RB-I00
Financiación: info:eu-repo/grantAgreement/ES/AEI/RYC2022-036627-I
Financiación: info:eu-repo/grantAgreement/ES/DGA/B29-23R
Financiación: info:eu-repo/grantAgreement/ES/DGA/LMP139-21
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/CB21-13-00087

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Inmunología (Dpto. Microb.Ped.Radio.Sal.Pú.)