Pulsed electric field-assisted extraction of carotenoids from fresh biomass of Rhodotorula glutinis

Martínez, J.M. (Universidad de Zaragoza) ; Delso, C. (Universidad de Zaragoza) ; Angulo, J. ; Álvarez, I. (Universidad de Zaragoza) ; Raso, J. (Universidad de Zaragoza)

Resumen: The aim of this study was to demonstrate the potential of PEF for inducing autolysis of R. glutinis, with the purpose of designing a more efficient and ecofriendly carotenoid extraction process: an extraction from fresh biomass, using cheaper, non-toxic, environmental-friendly solvents. Propidium iodide uptake and release of intracellular components revealed the irreversible electroporation of R. glutinis by PEF. Flow cytometry measurements detected morphological changes in PEF-treated R. glutinis cells during incubation caused by the autolysis triggering effect of electroporation. After submitting the fresh biomass to a PEF treatment (15 kV/cm, 150 µs) that irreversibly electroporated more than the 90% of the cells, ethanol proved ineffective for extracting carotenoids from fresh biomass of R. glutinis. However, after incubating the PEF-treated fresh biomass for 24 h at 20 °C in a pH 7 buffer, ca. 240 µg/g d.w. of carotenoids were recovered after 1 h of extraction in ethanol. The highest amount of carotenoids extracted (375 µg/g d.w.) from the PEF-treated cells of R. glutinis was obtained after having incubated them at 25 °C for 24 h in a medium of pH 8.0. The improvement in carotenoid extraction by incubating the R. glutinis cells after PEF treatment seems to be caused by PEF-triggered autolysis, which tends to disrupt the association of carotenoids with other molecules present in the cytoplasm, and causes a degradation of the cell wall.

Idioma: Inglés

DOI: 10.1016/j.ifset.2018.04.012

Año: 2018

Publicado en: INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES 47 (2018), 421-427
ISSN: 1466-8564

Factor impacto JCR: 4.085 (2018)

Categ. JCR: FOOD SCIENCE & TECHNOLOGY rank: 14 / 135 = 0.104 (2018) - Q1 - T1

Factor impacto SCIMAGO: 1.43 - Chemistry (miscellaneous) (Q1) - Industrial and Manufacturing Engineering (Q1) - Food Science (Q1)

Tipo y forma: Article (Published version)

Área (Departamento): Área Tecnología de Alimentos (Dpto. Produc.Animal Cienc.Ali.)