Isolation and characterization of milk exosomes for use in advanced therapies

Medel-Martinez, Ana ; Redrado-Osta, Ana ; Crespo-Barreda, Alejandra ; Sancho-Albero, Maria (Universidad de Zaragoza) ; Sánchez, Lourdes ; Sebastián, Víctor (Universidad de Zaragoza) ; Pardo, María ; de la Vieja, Antonio ; Martín-Duque, Pilar (Universidad de Zaragoza)

Resumen: Exosomes are cell-derived extracellular vesicles (EVs) with diameters between 30 and 120 nm. In recent years, several studies have evaluated the therapeutic potential of exosomes derived from different fluids due to their low immunogenicity and high biocompatibility. However, producing exosomes on a large scale is still challenging. One of the fluids from which they could be isolated in large quantities is milk. Moreover, regeneration is a well-known property of milk. The present work seeks to optimize a method for isolating exosomes from bovine and human milk, comparing different storage conditions and different extraction protocols. We found differences in the yield extraction associated with pre-storage milk conditions and observed some differences according to the processing agent. When we removed milk fat globules and added rennet before freezing, we obtained a cleaner final fraction. In summary, we attempted to optimize a rennet-based new milk–exosome isolation method and concluded that pre-treatment, followed by freezing of samples, yielded the best exosome population.

Idioma: Inglés

DOI: 10.3390/biom14070810

Año: 2024

Publicado en: Biomolecules 14, 7 (2024), 810 [17 pp.]
ISSN: 2218-273X

Financiación: info:eu-repo/grantAgreement/ES/ISCIII DTS21-00130
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/PI19-01007
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2021-125948OB-I00
Financiación: info:eu-repo/grantAgreement/ES/MICINN/RTI2018-099343-B-100

Tipo y forma: Article (Published version)

Área (Departamento): Área Estomatología (Dpto. Cirugía)
Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)