New insights into the function and molecular mechanisms of Ferredoxin-NADP+ reductase from Brucella ovis

Moreno, Andrea (Universidad de Zaragoza) ; Quereda-Moraleda, Isabel ; Lozano-Vallhonrat, Celia ; Buñuel-Escudero, María (Universidad de Zaragoza) ; Botha, Sabine ; Kupitz, Christopher ; Lisova, Stella ; Sierra, Ray ; Mariani, Valerio ; Schleissner, Pamela ; Gee, Leland B. ; Dörner, Katerina ; Schmidt, Christina ; Han, Huijong ; Kloos, Marco ; Smyth, Peter ; Valerio, Joana ; Schulz, Joachim ; de Wijn, Raphael ; Melo, Diogo V.M. ; Round, Adam ; Trost, Fabian ; Sobolev, Egor ; Juncheng, E. ; Sikorski, Marcin ; Bean, Richard ; Martínez-Júlvez, Marta (Universidad de Zaragoza) ; Martin-Garcia, Jose Manuel ; Medina, Milagros (Universidad de Zaragoza)

Resumen: Bacterial ferredoxin(flavodoxin)-NADP+ reductases (FPR) primarily catalyze the transfer of reducing equivalents from NADPH to ferredoxin (or flavodoxin) to provide low potential reducing equivalents for the oxidoreductive metabolism. In addition, they can be implicated in regulating reactive oxygen species levels. Here we assess the functionality of FPR from B. ovis to understand its potential roles in the bacteria physiology. We prove that this FPR is active with the endogenous [2Fe–2S] Fdx ferredoxin, exhibiting a KMFdx in the low micromolar range. At the molecular level, this study provides with the first structures of an FPR at room temperature obtained by serial femtosecond crystallography, envisaging increase in flexibility at both the adenine nucleotide moiety of FAD and the C-terminal tail. The produced microcrystals are in addition suitable for future mix-and-inject time-resolved studies with the NADP+/H coenzyme either at synchrotrons or XFELs. Furthermore, the study also predicts the ability of FPR to simultaneously interact with Fdx and NADP+/H.

Idioma: Inglés

DOI: 10.1016/j.abb.2024.110204

Año: 2024

Publicado en: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 762 (2024), 110204 [12 pp.]
ISSN: 0003-9861

Financiación: info:eu-repo/grantAgreement/ES/DGA/E35-23R
Financiación: info:eu-repo/grantAgreement/ES/DGA/LMP13_21
Financiación: info:eu-repo/grantAgreement/ES/MICINN/CNS2022-135713
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2022-136369NB-I00

Tipo y forma: Article (Published version)

Área (Departamento): Área Química Analítica (Dpto. Química Analítica)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)