Expansion under hypoxic conditions enhances the chondrogenic potential of equine bone marrow-derived mesenchymal stem cells

Ranera, B. ; Remacha, A. R. ; Álvarez-Arguedas, S. ; Castiella, T. (Universidad de Zaragoza) ; Vázquez, F. J. (Universidad de Zaragoza) ; Romero, A. (Universidad de Zaragoza) ; Zaragoza, P. (Universidad de Zaragoza) ; Martin-Burriel, I. (Universidad de Zaragoza) ; Rodellar, C. (Universidad de Zaragoza)

Resumen: Bone marrow-derived mesenchymal stem cells (BM-MSCs) are widely used in regenerative medicine in horses. Most of the molecular characterisations of BM-MSCs have been made at 20% O2, a higher oxygen level than the one surrounding the cells inside the bone marrow. The present work compares the lifespan and the tri-lineage potential of equine BM-MSCs expanded in normoxia (20% O2) and hypoxia (5% O2). No significant differences were found in long-term cultures for osteogenesis and adipogenesis between normoxic and hypoxic expanded BM-MSCs. An up-regulation of the chondrogenesis-related genes (COL2A1, ACAN, LUM, BGL, and COMP) and an increase of the extracellular sulphated glycosaminoglycan content were found in cells that were expanded under hypoxia. These results suggest that the expansion of BM-MSCs in hypoxic conditions enhances chondrogenesis in equine BM-MSCs.

Idioma: Inglés

DOI: 10.1016/j.tvjl.2012.06.008

Año: 2013

Publicado en: Veterinary Journal 195, 2 (2013), 248-251
ISSN: 1090-0233

Factor impacto JCR: 2.165 (2013)

Categ. JCR: VETERINARY SCIENCES rank: 11 / 131 = 0.084 (2013) - Q1 - T1

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Área Genética (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)
Área (Departamento): Area Anatomía Patológica (Dpto. Anat.Pat.Med.Leg.For.To.)