Effect of a Topical Formulation on Infective Viral Load in Lambs Naturally Infected with Orf Virus
Lacasta, Delia (Universidad de Zaragoza) ; Reina, Ramses ; Ruiz de Arcaute, Marta (Universidad de Zaragoza) ; Ferrer, Luis Miguel (Universidad de Zaragoza) ; Benito, Alfredo Angel ; Tejedor, Maria Teresa (Universidad de Zaragoza) ; Echeverria, Irache ; Ruiz, Hector (Universidad de Zaragoza) ; Martinez Cardenas, Silvia ; Windsor, Peter Andrew
Resumen: Introduction: Orf is a highly contagious eruptive viral disease of the skin and mucosa of sheep and goats. Although vaccination with live or attenuated orf virus is the preferred option for disease control, the vaccine is unavailable in many countries. Treatment of orf lesions involves standard hygiene and in numerous cases, management of presumptive secondary infections with antibiotics, increasing risks of antimicrobial resistance (AMR). The wound dressing formulation Tri-Solfen® containing two local anaesthetics (lignocaine and bupivacaine), adrenaline and an antiseptic (cetrimide) in a gel formulation was developed for pain relief in sheep undergoing surgical husbandry procedures in Australia. Recently, TS therapy was found to reduce suffering and enhance recovery in cattle and buffalo with oral and skin lesions due to foot-and-mouth disease (FMD) virus infection. It was noted that TS has a low pH and is potentially viricidal, potentially aiding disease control.
Methods: One-month-old lambs (n=14), naturally infected with orf, were recruited from a farm during a natural outbreak of the disease. The animals were selected at the early stages of the infection and randomly divided into two cohorts: Group A (n=11) treated with the topical wound gel formulation (TS); and Group B (n=3) an untreated control group. Swabs were obtained before treatment (T0) and on days one (T1), 3 (T2) and 5 (T3) post-treatment, then submitted to direct DNA extraction with real-time PCR quantification, plus incubation with primary tissue cultures from ovine skin fibroblasts (OSF) and T-immortalized goat embryonic fibroblasts (TIGEF).
Results: Although no significant differences were found in the clinical progression of the lesions and PCR quantification (p=0.722) between these small cohorts, there was a significant difference (p< 0.05) in reduction in infective viral load between the groups when assessed in OSF cell cultures between T0 and T3.
Conclusion: These preliminary findings suggest that treatment of early stage lesions with this TS may reduce the infective viral load present in orf lesions.
Idioma: Inglés
DOI: 10.2147/VMRR.S306355
Año: 2021
Publicado en: Veterinary medicine 12 (2021), 149-158
ISSN: 2230-2034
Financiación: info:eu-repo/grantAgreement/ES/DGA/A15-17R
Tipo y forma: Article (Published version)
Área (Departamento): Área Genética (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes.
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Methods: One-month-old lambs (n=14), naturally infected with orf, were recruited from a farm during a natural outbreak of the disease. The animals were selected at the early stages of the infection and randomly divided into two cohorts: Group A (n=11) treated with the topical wound gel formulation (TS); and Group B (n=3) an untreated control group. Swabs were obtained before treatment (T0) and on days one (T1), 3 (T2) and 5 (T3) post-treatment, then submitted to direct DNA extraction with real-time PCR quantification, plus incubation with primary tissue cultures from ovine skin fibroblasts (OSF) and T-immortalized goat embryonic fibroblasts (TIGEF).
Results: Although no significant differences were found in the clinical progression of the lesions and PCR quantification (p=0.722) between these small cohorts, there was a significant difference (p< 0.05) in reduction in infective viral load between the groups when assessed in OSF cell cultures between T0 and T3.
Conclusion: These preliminary findings suggest that treatment of early stage lesions with this TS may reduce the infective viral load present in orf lesions.
Idioma: Inglés
DOI: 10.2147/VMRR.S306355
Año: 2021
Publicado en: Veterinary medicine 12 (2021), 149-158
ISSN: 2230-2034
Financiación: info:eu-repo/grantAgreement/ES/DGA/A15-17R
Tipo y forma: Article (Published version)
Área (Departamento): Área Genética (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. Exportado de SIDERAL (2025-01-23-14:46:49)
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Record created 2025-01-23, last modified 2025-01-23