Structural and functional insights into UDP-N-acetylglucosamine-enolpyruvate reductase (MurB) from Brucella ovis

Minjárez-Sáenz, Martha ; Rivero, Maribel (Universidad de Zaragoza) ; Correa-Pérez, Víctor (Universidad de Zaragoza) ; Boneta, Sergio ; Suárez, Paula ; Polo, Víctor (Universidad de Zaragoza) ; Sadeghi, Sheila J. ; Yruela, Inmaculada ; Martínez-Júlvez, Marta (Universidad de Zaragoza) ; Medina, Milagros (Universidad de Zaragoza)

Resumen: The peptidoglycan biosynthetic pathway involves a series of enzymatic reactions in which UDP-N-acetylglucosamine-enolpyruvate reductase (MurB) plays a crucial role in catalyzing the conversion of UDP-N-acetylglucosamine-enolpyruvate (UNAGEP) to UDP-N-acetylmuramic acid. This reaction relies on NADPH and FAD and, since MurB is not found in eukaryotes, it is an attractive target for the development of antimicrobials. MurB from Brucella ovis, the causative agent of brucellosis in sheep, is characterized here. The FAD cofactor in MurB of B. ovis is reduced to the hydroquinone state without semiquinone stabilization with an estimated Eox/hq of −260 mV. MurB from B. ovis catalyzes the oxidation of NADPH in a slow process that is positively influenced by the presence of the second product, UNAGEP. The crystallographic structure of the MurBox:UNAGEP complex confirms its folding into three domains and the binding of UNAGEP, positioning its enolpyruvyl group for hydride transfer from FAD. MurB shows a complex thermal unfolding pathway that is influenced by UNAGEP and NADP+, confirming its ability to bind both molecules. Molecular dynamics (MD) simulations predict that the nicotinamide of NADP+ is more stable at the active site than the enolpyruvyl of UNAGEP, and suggests that MurB can simultaneously accommodate NADPH and UNAGEP in the substrate channel, increasing overall protein-ligand flexibility. Sequence and evolutionary analyses show that MurB from B. ovis conserves all motifs predicted to be involved in catalysis within the Type IIa family.

Idioma: Inglés

DOI: 10.1016/j.abb.2025.110288

Año: 2025

Publicado en: ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 765 (2025), 110288 [19 pp.]
ISSN: 0003-9861

Financiación: info:eu-repo/grantAgreement/ES/DGA/E35-23R
Financiación: info:eu-repo/grantAgreement/ES/DGA/LMP13_21
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2022-136369NB-I00

Tipo y forma: Article (Published version)

Área (Departamento): Área Química Física (Dpto. Química Física)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)