Variety, maturity stage and postharvest storage time effect on Mal d 3 allergen from apple (Malus domestica)

Esteban-Sanz, Clara (Universidad de Zaragoza) ; Tobajas, Ana P. (Universidad de Zaragoza) ; Gracia, Ana P. (Universidad de Zaragoza) ; Civera, Alba ; Sánchez, Lourdes (Universidad de Zaragoza) ; Arias, Esther (Universidad de Zaragoza) ; Pérez, María D. (Universidad de Zaragoza)

Resumen: Fruit belonging to Rosaceae family, which includes apples, is responsible for 60 % of fruit allergies. Mal d 3, a member of the lipid transfer proteins, is the most allergenic protein of apple. Levels of total soluble protein and Mal d 3 were determined in apple peel as a function of variety, maturity stage and postharvest storage time under controlled atmosphere conditions, using a previously developed specific sandwich ELISA. Mal d 3 content differed among varieties, ranging from 13.6 mg kg−1 in ‘Golden Delicious’ to 23.6 mg kg−1 in ‘Verde Doncella’. No significant influence of the maturity stage on Mal d 3 concentration was found. Over a 7-month period of storage under controlled atmosphere, the levels of Mal d 3 decreased about 1.6–2.3 times. No correlation was found between Mal d 3 concentration and total soluble protein in apple peel samples. A better understanding of how Mal d 3 concentration is affected by pre and postharvest factors could assist to decrease the allergen load of apple.

Idioma: Inglés

DOI: 10.1016/j.postharvbio.2025.113512

Año: 2025

Publicado en: Postharvest Biology and Technology 225 (2025), 113512 [8 pp.]
ISSN: 0925-5214

Financiación: info:eu-repo/grantAgreement/ES/DGA/A20-23R

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Tecnología de Alimentos (Dpto. Produc.Animal Cienc.Ali.)