Supercomplex assembly determines electron flux in the mitochondrial electron transport chain

Lapuente-Brun, E. ; Moreno-Loshuertos, R. (Universidad de Zaragoza) ; Acín-Pérez, R. ; Latorre-Pellicer, A. ; Colas, C. ; Balsa, E. ; Perales-Clemente, E. ; Quirós, P.M. ; Calvo, E. ; Rodríguez-Hernández, M.A. ; Navas, P. ; Cruz, R. ; Carracedo, Á. ; López-Otín, C. ; Pérez-Martos, A. ; Fernández-Silva, P. (Universidad de Zaragoza) ; Fernández-Vizarra, E. ; Enríquez, J.A.

Resumen: The textbook description of mitochondrial respiratory complexes (RCs) views them as free-moving entities linked by the mobile carriers coenzyme Q (CoQ) and cytochrome c (cyt c). This model (known as the fluid model) is challenged by the proposal that all RCs except complex II can associate in supercomplexes (SCs). The proposed SCs are the respirasome (complexes I, III, and IV), complexes I and III, and complexes III and IV. The role of SCs is unclear, and their existence is debated. By genetic modulation of interactions between complexes I and III and III and IV, we show that these associations define dedicated CoQ and cyt c pools and that SC assembly is dynamic and organizes electron flux to optimize the use of available substrates.

Idioma: Inglés

DOI: 10.1126/science.1230381

Año: 2013

Publicado en: Science 340, 6140 (2013), 1567-1570
ISSN: 0036-8075

Factor impacto JCR: 31.477 (2013)

Categ. JCR: MULTIDISCIPLINARY SCIENCES rank: 2 / 56 = 0.036 (2013) - Q1 - T1

Tipo y forma: Article (Published version)

Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)