Isolation of biogenetically competent mitochondria from mammalian tissues and cultured cells.

Fernandez Vizarra, E. ; Lopez Perez, M. J (Universidad de Zaragoza) ; Enriquez, J. A (Universidad de Zaragoza)

Resumen: This article describes a quick basic method adapted for the purification of mammalian mitochondria from different sources. The organelles obtained using this protocol are suitable for the investigation of biogenetic activities such as enzyme activity, mtDNA, mtRNA, mitochondrial protein synthesis, and mitochondrial tRNA aminoacylation. In addition, these mitochondria are capable of efficient protein import and the investigation of mtDNA/protein interactions by DNA footprinting is also possible

Idioma: Inglés

DOI: 10.1016/S1046-2023(02)00034-8

Año: 2002

Publicado en: METHODS 26, 4 (2002), 292-297
ISSN: 1046-2023

Factor impacto JCR: 2.233 (2002)

Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 131 / 262 = 0.5 (2002) - Q2 - T2
Categ. JCR: BIOCHEMICAL RESEARCH METHODS rank: 15 / 45 = 0.333 (2002) - Q2 - T2

Tipo y forma: Article (Published version)

Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)