Systematic identification of novel glycosidic aromatic precursors in winemaking grapes via preparative LC fractionation, target GC and non-targeted UHPLC-MS analysis

González-Martínez, Belén (Universidad de Zaragoza) ; Sánchez-Acevedo, Elayma ; de-la-Fuente-Blanco, Arancha (Universidad de Zaragoza) ; Ontañón, Ignacio (Universidad de Zaragoza) ; Lopez, Ricardo (Universidad de Zaragoza) ; Ferreira, Vicente (Universidad de Zaragoza)

Resumen: Grapes used in winemaking contain non-volatile precursors that, through chemical reactions and rearrangements, release key aromatic compounds essential to wine aroma. This study aims to identify aroma precursors in extracts from ‘Garnacha’ grapes combining sequential C18, semi-preparative chromatographies (size exclusion + normal phase), target SPME-GC–MS analysis of the hydrolysed fractions and untargeted UHPLC-MS of the original fractions. Ninety-nine fractions were obtained, 92 of which released aroma upon hydrolysis. Targeted aroma compounds (terpenes, norisoprenoids, volatile phenols, vanillins and ethyl cinnamates) were detected in the hydrolysates of 55 fractions. Identification of precursors was based on: a) exact molecular mass, b) MS/MS spectrum, and c) correlation between the precursor and aroma signals in UHPLC-MS and GC–MS in the original and hydrolysed fractions, respectively. Overall, 185 precursors were identified, 98 for the first time, including 94 disaccharides, 57 trisaccharides and 34 monosaccharides from: terpenes (83), volatile phenols (30), cinnamic acid derivatives (22), norisoprenoids (21), vanillins (19), ethyl leucate (6) and furaneol (4). This work represents a significant advancement in the evaluation of the potential aromatic quality of winemaking grapes using UHPLC-MS.

Idioma: Inglés

DOI: 10.1016/j.foodchem.2025.147361

Año: 2025

Publicado en: Food Chemistry 499 (2025), 147361 [21 pp.]
ISSN: 0308-8146

Financiación: info:eu-repo/grantAgreement/ES/AEI/PID2021-126031OB-C21
Financiación: info:eu-repo/grantAgreement/ES/DGA/T29
Financiación: info:eu-repo/grantAgreement/ES/MCINN/PRE2018-084968

Tipo y forma: Article (Published version)

Área (Departamento): Área Química Analítica (Dpto. Química Analítica)