Co‐immobilized Phosphorylated Cofactors and Enzymes as Self‐Sufficient Heterogeneous Biocatalysts for Chemical Processes
Velasco-Lozano, Susana ; Benítez-Mateos, Ana I. ; López-Gallego, Fernando
Resumen: Enzyme cofactors play a major role in biocatalysis, as many enzymes require them to catalyze highly valuable reactions in organic synthesis. However, the cofactor recycling is often a hurdle to implement enzymes at the industrial level. The fabrication of heterogeneous biocatalysts co‐immobilizing phosphorylated cofactors (PLP, FAD+, and NAD+) and enzymes onto the same solid material is reported to perform chemical reactions without exogeneous addition of cofactors in aqueous media. In these self‐sufficient heterogeneous biocatalysts, the immobilized enzymes are catalytically active and the immobilized cofactors catalytically available and retained into the solid phase for several reaction cycles. Finally, we have applied a NAD+‐dependent heterogeneous biocatalyst to continuous flow asymmetric reduction of prochiral ketones, thus demonstrating the robustness of this approach for large scale biotransformations.
Idioma: Inglés
DOI: 10.1002/anie.201609758
Año: 2017
Publicado en: Angewandte Chemie (International ed.) 56, 3 (2017), 771-775
ISSN: 1433-7851
Factor impacto JCR: 12.102 (2017)
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 14 / 169 = 0.083 (2017) - Q1 - T1
Factor impacto SCIMAGO: 6.155 - Chemistry (miscellaneous) (Q1) - Catalysis (Q1)
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BIO2014-61838-EXP
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BIO2015-69887-R
Tipo y forma: Article (Published version)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes.
Exportado de SIDERAL (2026-01-27-15:01:22)
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Idioma: Inglés
DOI: 10.1002/anie.201609758
Año: 2017
Publicado en: Angewandte Chemie (International ed.) 56, 3 (2017), 771-775
ISSN: 1433-7851
Factor impacto JCR: 12.102 (2017)
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 14 / 169 = 0.083 (2017) - Q1 - T1
Factor impacto SCIMAGO: 6.155 - Chemistry (miscellaneous) (Q1) - Catalysis (Q1)
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BIO2014-61838-EXP
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BIO2015-69887-R
Tipo y forma: Article (Published version)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. Exportado de SIDERAL (2026-01-27-15:01:22)
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Record created 2026-01-27, last modified 2026-01-27