Co‐immobilized Phosphorylated Cofactors and Enzymes as Self‐Sufficient Heterogeneous Biocatalysts for Chemical Processes

Velasco-Lozano, Susana ; Benítez-Mateos, Ana I. ; López-Gallego, Fernando

Resumen: Enzyme cofactors play a major role in biocatalysis, as many enzymes require them to catalyze highly valuable reactions in organic synthesis. However, the cofactor recycling is often a hurdle to implement enzymes at the industrial level. The fabrication of heterogeneous biocatalysts co‐immobilizing phosphorylated cofactors (PLP, FAD+, and NAD+) and enzymes onto the same solid material is reported to perform chemical reactions without exogeneous addition of cofactors in aqueous media. In these self‐sufficient heterogeneous biocatalysts, the immobilized enzymes are catalytically active and the immobilized cofactors catalytically available and retained into the solid phase for several reaction cycles. Finally, we have applied a NAD+‐dependent heterogeneous biocatalyst to continuous flow asymmetric reduction of prochiral ketones, thus demonstrating the robustness of this approach for large scale biotransformations.

Idioma: Inglés

DOI: 10.1002/anie.201609758

Año: 2017

Publicado en: Angewandte Chemie (International ed.) 56, 3 (2017), 771-775
ISSN: 1433-7851

Factor impacto JCR: 12.102 (2017)

Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 14 / 169 = 0.083 (2017) - Q1 - T1

Factor impacto SCIMAGO: 6.155 - Chemistry (miscellaneous) (Q1) - Catalysis (Q1)

Financiación: info:eu-repo/grantAgreement/ES/MINECO/BIO2014-61838-EXP
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BIO2015-69887-R

Tipo y forma: Article (Published version)