Characterization of Fibrin and Collagen Gels for Engineering Wound Healing Models
Moreno-Arotzena , Oihana ; Meier, Johann G ; del Amo, Cristina (Universidad de Zaragoza) ; García-Aznar, José Manuel (Universidad de Zaragoza)
Resumen: Hydrogels are used for 3D in vitro assays and tissue engineering and regeneration purposes. For a thorough interpretation of this technology, an integral biomechanical characterization of the materials is required. In this work, we characterize the mechanical and functional behavior of two specific hydrogels that play critical roles in wound healing, collagen and fibrin. A coherent and complementary characterization was performed using a
generalized and standard composition of each hydrogel and a combination of techniques. Microstructural analysis was performed by scanning electron microscopy and confocal reflection imaging. Permeability was measured using a microfluidic-based experimental set-up, and mechanical responses were analyzed by rheology. We measured a pore size of 2.84 and 1.69 µm for collagen and fibrin, respectively. Correspondingly, the permeability of the gels was 1.00·10-12 and 5.73·10-13 m2. The shear modulus in the linear viscoelastic regime was 15 Pa for collagen and 300 Pa for fibrin. The gels exhibited strain-hardening behavior at ca. 10% and 50% strain for fibrin and collagen, respectively. This consistent biomechanical characterization provides a detailed and robust starting point for different 3D in vitro bioapplications, such as collagen and/or fibrin gels. These features may have major implications for 3D cellular behavior by inducing divergent microenvironmental cues.
Idioma: Inglés
DOI: 10.3390/ma8041636
Año: 2015
Publicado en: MATERIALS 8 (2015), 1636-1651
ISSN: 1996-1944
Factor impacto JCR: 2.728 (2015)
Categ. JCR: MATERIALS SCIENCE, MULTIDISCIPLINARY rank: 63 / 271 = 0.232 (2015) - Q1 - T1
Factor impacto SCIMAGO: 0.83 - Materials Science (miscellaneous) (Q1)
Financiación: info:eu-repo/grantAgreement/EUR/FP7/ERC2012-StG-306751
Financiación: info:eu-repo/grantAgreement/ES/MINECO/DPI2012-38090-C03-01
Tipo y forma: Article (Published version)
Área (Departamento): Área Mec.Med.Cont. y Teor.Est. (Dpto. Ingeniería Mecánica)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
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generalized and standard composition of each hydrogel and a combination of techniques. Microstructural analysis was performed by scanning electron microscopy and confocal reflection imaging. Permeability was measured using a microfluidic-based experimental set-up, and mechanical responses were analyzed by rheology. We measured a pore size of 2.84 and 1.69 µm for collagen and fibrin, respectively. Correspondingly, the permeability of the gels was 1.00·10-12 and 5.73·10-13 m2. The shear modulus in the linear viscoelastic regime was 15 Pa for collagen and 300 Pa for fibrin. The gels exhibited strain-hardening behavior at ca. 10% and 50% strain for fibrin and collagen, respectively. This consistent biomechanical characterization provides a detailed and robust starting point for different 3D in vitro bioapplications, such as collagen and/or fibrin gels. These features may have major implications for 3D cellular behavior by inducing divergent microenvironmental cues.
Idioma: Inglés
DOI: 10.3390/ma8041636
Año: 2015
Publicado en: MATERIALS 8 (2015), 1636-1651
ISSN: 1996-1944
Factor impacto JCR: 2.728 (2015)
Categ. JCR: MATERIALS SCIENCE, MULTIDISCIPLINARY rank: 63 / 271 = 0.232 (2015) - Q1 - T1
Factor impacto SCIMAGO: 0.83 - Materials Science (miscellaneous) (Q1)
Financiación: info:eu-repo/grantAgreement/EUR/FP7/ERC2012-StG-306751
Financiación: info:eu-repo/grantAgreement/ES/MINECO/DPI2012-38090-C03-01
Tipo y forma: Article (Published version)
Área (Departamento): Área Mec.Med.Cont. y Teor.Est. (Dpto. Ingeniería Mecánica)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. Exportado de SIDERAL (2021-01-21-08:15:30)
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Record created 2015-10-28, last modified 2021-01-21