Single nucleotide polymorphism (SNP) analysis used for the phylogeny of the Mycobacterium tuberculosis complex based on a pyrosequencing assay
Cabal, A. ; Strunk, M. ; Domínguez, J. ; Lezcano, M.A. ; Vitoria, M.A. (Universidad de Zaragoza) ; Ferrero, M. ; Martín, C. ; Iglesias, M.J. (Universidad de Zaragoza) ; Samper, S.
Resumen: Background
Different polymorphisms have been described as markers to classify the lineages of the Mycobacterium tuberculosis complex. The analysis of nine single nucleotide polymorphisms (SNPs) was used to describe seven SNPs cluster groups (SCGs). We attempted to classify those strains that could not been categorized into lineages by the genotyping methods used in the routine testing.
Results
The M. tuberculosis complex isolates collected in 2010 in our region were analysed. A new method based on multiplex-PCRs and pyrosequencing to analyse these SNPs was designed. For the pyrosequencing assay nine SNPs that defined the seven SCGs were selected from the literature: 1977, 74092, 105139, 232574, 311613, 913274, 2460626, 3352929 and gyrA95. In addition, SNPs in katG463, mgtC182, Ag85C103 and RDRio deletion were detected.
Idioma: Inglés
DOI: 10.1186/1471-2180-14-21
Año: 2014
Publicado en: BMC MICROBIOLOGY 14, 21 (2014), [9 pp.]
ISSN: 1471-2180
Factor impacto JCR: 2.729 (2014)
Categ. JCR: MICROBIOLOGY rank: 51 / 119 = 0.429 (2014) - Q2 - T2
Financiación: info:eu-repo/grantAgreement/ES/FIS/FIS09-051
Financiación: info:eu-repo/grantAgreement/ES/FIS/FIS12/1970
Tipo y forma: Article (Published version)
Área (Departamento): Área Microbiología (Dpto. Microb.Med.Pr.,Sal.Públ.)
Área (Departamento): Área Medic.Prevent.Salud Públ. (Dpto. Microb.Med.Pr.,Sal.Públ.)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. If you remix, transform, or build upon the material, you may not distribute the modified material.
Exportado de SIDERAL (2021-06-18-11:14:21)
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Different polymorphisms have been described as markers to classify the lineages of the Mycobacterium tuberculosis complex. The analysis of nine single nucleotide polymorphisms (SNPs) was used to describe seven SNPs cluster groups (SCGs). We attempted to classify those strains that could not been categorized into lineages by the genotyping methods used in the routine testing.
Results
The M. tuberculosis complex isolates collected in 2010 in our region were analysed. A new method based on multiplex-PCRs and pyrosequencing to analyse these SNPs was designed. For the pyrosequencing assay nine SNPs that defined the seven SCGs were selected from the literature: 1977, 74092, 105139, 232574, 311613, 913274, 2460626, 3352929 and gyrA95. In addition, SNPs in katG463, mgtC182, Ag85C103 and RDRio deletion were detected.
Idioma: Inglés
DOI: 10.1186/1471-2180-14-21
Año: 2014
Publicado en: BMC MICROBIOLOGY 14, 21 (2014), [9 pp.]
ISSN: 1471-2180
Factor impacto JCR: 2.729 (2014)
Categ. JCR: MICROBIOLOGY rank: 51 / 119 = 0.429 (2014) - Q2 - T2
Financiación: info:eu-repo/grantAgreement/ES/FIS/FIS09-051
Financiación: info:eu-repo/grantAgreement/ES/FIS/FIS12/1970
Tipo y forma: Article (Published version)
Área (Departamento): Área Microbiología (Dpto. Microb.Med.Pr.,Sal.Públ.)
Área (Departamento): Área Medic.Prevent.Salud Públ. (Dpto. Microb.Med.Pr.,Sal.Públ.)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. If you remix, transform, or build upon the material, you may not distribute the modified material. Exportado de SIDERAL (2021-06-18-11:14:21)
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Este artículo se encuentra en las siguientes colecciones:
Articles > Artículos por área > Medicina Preventiva y Salud Pública
Articles > Artículos por área > Microbiología
Record created 2015-10-28, last modified 2021-06-18