Fibroblast migration in 3D is controlled by haptotaxis in a non-muscle myosin II-dependent manner

Moreno-Arotzena, O. ; Borau, C. (Universidad de Zaragoza) ; Movilla, N. ; Vicente-Manzanares, M. ; García-Aznar, J.M. (Universidad de Zaragoza)

Resumen: Cell migration in 3D is a key process in many physiological and pathological processes. Although valuable knowledge has been accumulated through analysis of various 2D models, some of these insights are not directly applicable to migration in 3D. In this study, we have confined biomimetic hydrogels within microfluidic platforms in the presence of a chemoattractant (platelet-derived growth fac- tor-BB). We have characterized the migratory responses of human fibroblasts within them, particularly focusing on the role of non-muscle myosin II. Our results indicate a prominent role for myosin II in the integration of chemo- tactic and haptotactic migratory responses of fibroblasts in 3D confined environments.

Idioma: Inglés

DOI: 10.1007/s10439-015-1343-2

Año: 2015

Publicado en: Annals of Biomedical Engineering 43, 12 (2015), 3025-3039
ISSN: 0090-6964

Factor impacto JCR: 2.887 (2015)

Categ. JCR: ENGINEERING, BIOMEDICAL rank: 16 / 76 = 0.211 (2015) - Q1 - T1

Factor impacto SCIMAGO: 1.2 - Biomedical Engineering (Q1)

Financiación: info:eu-repo/grantAgreement/EUR/FP7/ERC2012-StG-306751
Financiación: info:eu-repo/grantAgreement/ES/MINECO/DPI2012-38090-C03-01
Financiación: info:eu-repo/grantAgreement/ES/MINECO/SAF2011-24953

Tipo y forma: Article (Published version)

Área (Departamento): Área Mec.Med.Cont. y Teor.Est. (Dpto. Ingeniería Mecánica)