Antimicrobial resistance, virulence factorsand genetic lineages of hospital-onsetmethicillin-resistant Staphylococcus aureus isolates detected in a hospital in Zaragoza
González-Domínguez, M ; Seral, C (Universidad de Zaragoza) ; Potel, C ; Constenla, L ; Algarate, S ; Gude, MJ ; Alvarez, M ; Castillo FJ (Universidad de Zaragoza)
Resumen: Introduction
MRSA population dynamics is undergoing significant changes, and for this reason it is important to know which clones are circulating in our nosocomial environment.
Materials and methods
A total of 118 MRSA isolates were collected from clinical samples from patients with previous hospital or healthcare contact (named as hospital-onset MRSA (HO-MRSA)) during a one year period. Susceptibility testing was performed by disk diffusion and microdilution. The presence of resistance genes and virulence factors were tested by PCR. All isolates were typed by SCCmec, spa and agr typing. PFGE and MLST were applied to a selection of them.
Results
Eighty-three HO-MRSA isolates (70.3%) were resistant to any antibiotic included in the macrolide–lincosamide–streptogramin B group. Among these isolates, the M phenotype was the most frequent (73.5%). One hundred and seven of HO-MRSA isolates (90.7%) showed aminoglycoside resistance. The combination aac(6')-Ie-aph(2")-Ia + ant(4')-Ia genes was the most frequent (22.4%). Tetracycline resistance rates in HO-MRSA isolates were low (3.4%), although a high level of mupirocin resistance was observed (25.4%). Most of the HO-MRSA isolates (approximately 90%) showed SCCmec type IVc and agr type II. Fifteen unrelated pulsotypes were identified. CC5 was the most prevalent (88.1%), followed by CC8 (5.9%), CC22 (2.5%), CC398 (2.5%) and CC1 (0.8%).
Conclusion
CC5/ST125/t067 lineage was the most frequent. This lineage was related to aminoglycoside resistance, and to a lesser extent, with macrolide resistance. The presence of international clones as EMRSA-15 (CC22/ST22), European clones as CC5/ST228, community clones related to CC1 or CC8 and livestock associated clones, as CC398, were observed in a low percentage.
Idioma: Inglés
DOI: 10.1016/j.eimc.2015.01.015
Año: 2015
Publicado en: Enfermedades Infecciosas y Microbiologia Clinica 33, 9 (2015), 590-596
ISSN: 0213-005X
Factor impacto JCR: 1.53 (2015)
Categ. JCR: INFECTIOUS DISEASES rank: 59 / 83 = 0.711 (2015) - Q3 - T3
Categ. JCR: MICROBIOLOGY rank: 97 / 123 = 0.789 (2015) - Q4 - T3
Factor impacto SCIMAGO: 0.378 - Microbiology (medical) (Q3)
Financiación: info:eu-repo/grantAgreement/ES/DGA/B24-211130
Tipo y forma: Artículo (PostPrint)
Área (Departamento): Área Microbiología (Dpto. Microb.Med.Pr.,Sal.Públ.)
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Exportado de SIDERAL (2021-01-21-10:57:33)
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MRSA population dynamics is undergoing significant changes, and for this reason it is important to know which clones are circulating in our nosocomial environment.
Materials and methods
A total of 118 MRSA isolates were collected from clinical samples from patients with previous hospital or healthcare contact (named as hospital-onset MRSA (HO-MRSA)) during a one year period. Susceptibility testing was performed by disk diffusion and microdilution. The presence of resistance genes and virulence factors were tested by PCR. All isolates were typed by SCCmec, spa and agr typing. PFGE and MLST were applied to a selection of them.
Results
Eighty-three HO-MRSA isolates (70.3%) were resistant to any antibiotic included in the macrolide–lincosamide–streptogramin B group. Among these isolates, the M phenotype was the most frequent (73.5%). One hundred and seven of HO-MRSA isolates (90.7%) showed aminoglycoside resistance. The combination aac(6')-Ie-aph(2")-Ia + ant(4')-Ia genes was the most frequent (22.4%). Tetracycline resistance rates in HO-MRSA isolates were low (3.4%), although a high level of mupirocin resistance was observed (25.4%). Most of the HO-MRSA isolates (approximately 90%) showed SCCmec type IVc and agr type II. Fifteen unrelated pulsotypes were identified. CC5 was the most prevalent (88.1%), followed by CC8 (5.9%), CC22 (2.5%), CC398 (2.5%) and CC1 (0.8%).
Conclusion
CC5/ST125/t067 lineage was the most frequent. This lineage was related to aminoglycoside resistance, and to a lesser extent, with macrolide resistance. The presence of international clones as EMRSA-15 (CC22/ST22), European clones as CC5/ST228, community clones related to CC1 or CC8 and livestock associated clones, as CC398, were observed in a low percentage.
Idioma: Inglés
DOI: 10.1016/j.eimc.2015.01.015
Año: 2015
Publicado en: Enfermedades Infecciosas y Microbiologia Clinica 33, 9 (2015), 590-596
ISSN: 0213-005X
Factor impacto JCR: 1.53 (2015)
Categ. JCR: INFECTIOUS DISEASES rank: 59 / 83 = 0.711 (2015) - Q3 - T3
Categ. JCR: MICROBIOLOGY rank: 97 / 123 = 0.789 (2015) - Q4 - T3
Factor impacto SCIMAGO: 0.378 - Microbiology (medical) (Q3)
Financiación: info:eu-repo/grantAgreement/ES/DGA/B24-211130
Tipo y forma: Artículo (PostPrint)
Área (Departamento): Área Microbiología (Dpto. Microb.Med.Pr.,Sal.Públ.)
Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace. No puede utilizar el material para una finalidad comercial. Si remezcla, transforma o crea a partir del material, no puede difundir el material modificado.Exportado de SIDERAL (2021-01-21-10:57:33)
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Registro creado el 2016-04-07, última modificación el 2021-01-21