A unified framework based on the binding polynomial for characterizing biological systems by isothermal titration calorimetry
Vega, S. ; Abian, O. ; Velazquez-Campoy, A. (Universidad de Zaragoza)
Resumen: Isothermal titration calorimetry (ITC) has become the gold-standard technique for studying binding processes due to its high precision and sensitivity, as well as its capability for the simultaneous determination of the association equilibrium constant, the binding enthalpy and the binding stoichiometry. The current widespread use of ITC for biological systems has been facilitated by technical advances and the availability of commercial calorimeters. However, the complexity of data analysis for non-standard models is one of the most significant drawbacks in ITC. Many models for studying macromolecular interactions can be found in the literature, but it looks like each biological system requires specific modeling and data analysis approaches. The aim of this article is to solve this lack of unity and provide a unified methodological framework for studying binding interactions by ITC that can be applied to any experimental system. The apparent complexity of this methodology, based on the binding polynomial, is overcome by its easy generalization to complex systems.
Idioma: Inglés
DOI: 10.1016/j.ymeth.2014.09.010
Año: 2015
Publicado en: METHODS 76 (2015), 99-115
ISSN: 1046-2023
Factor impacto JCR: 3.503 (2015)
Categ. JCR: BIOCHEMICAL RESEARCH METHODS rank: 18 / 77 = 0.234 (2015) - Q1 - T1
Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 99 / 289 = 0.343 (2015) - Q2 - T2
Factor impacto SCIMAGO: 2.367 - Molecular Biology (Q1) - Biochemistry, Genetics and Molecular Biology (miscellaneous) (Q1)
Financiación: info:eu-repo/grantAgreement/ES/DGA/B01
Financiación: info:eu-repo/grantAgreement/ES/DGA/B89
Financiación: info:eu-repo/grantAgreement/ES/FIS/PI10-00186
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/CP07-00289
Financiación: info:eu-repo/grantAgreement/ES/MICINN/BFU2010-19451
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PTA2009-2341-I
Tipo y forma: Article (PostPrint)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. If you remix, transform, or build upon the material, you may not distribute the modified material.
Exportado de SIDERAL (2021-01-21-10:56:15)
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Idioma: Inglés
DOI: 10.1016/j.ymeth.2014.09.010
Año: 2015
Publicado en: METHODS 76 (2015), 99-115
ISSN: 1046-2023
Factor impacto JCR: 3.503 (2015)
Categ. JCR: BIOCHEMICAL RESEARCH METHODS rank: 18 / 77 = 0.234 (2015) - Q1 - T1
Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 99 / 289 = 0.343 (2015) - Q2 - T2
Factor impacto SCIMAGO: 2.367 - Molecular Biology (Q1) - Biochemistry, Genetics and Molecular Biology (miscellaneous) (Q1)
Financiación: info:eu-repo/grantAgreement/ES/DGA/B01
Financiación: info:eu-repo/grantAgreement/ES/DGA/B89
Financiación: info:eu-repo/grantAgreement/ES/FIS/PI10-00186
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/CP07-00289
Financiación: info:eu-repo/grantAgreement/ES/MICINN/BFU2010-19451
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PTA2009-2341-I
Tipo y forma: Article (PostPrint)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. If you remix, transform, or build upon the material, you may not distribute the modified material. Exportado de SIDERAL (2021-01-21-10:56:15)
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Articles > Artículos por área > Bioquímica y Biología Molecular
Record created 2017-05-31, last modified 2021-01-21