Toll-like receptors 2 and 4 modulate intestinal IL-10 differently in ileum and colon

Latorre, E. (Universidad de Zaragoza) ; Layunta, E. ; Grasa, L. (Universidad de Zaragoza) ; Pardo, J. (Universidad de Zaragoza) ; García, S. (Universidad de Zaragoza) ; Alcalde, A. I. ; Mesonero, J. E. (Universidad de Zaragoza)

Resumen: Background: Inflammatory bowel diseases are consequence of an intestinal homeostasis breakdown in which innate immune dysregulation is implicated. Toll-like receptor (TLR)2 and TLR4 are immune recognition receptors expressed in the intestinal epithelium, the first physical-physiological barrier for microorganisms, to inform the host of the presence of Gram-positive and Gram-negative organisms. Interleukin (IL)-10 is an essential anti-inflammatory cytokine that contributes to maintenance of intestinal homeostasis. Aim: Our main aim was to investigate intestinal IL-10 synthesis and release, and whether TLR2 and TLR4 are determinants of IL-10 expression in the intestinal tract. Methods: We used Caco-2 cell line as an enterocyte-like cell model, and also ileum and colon from mice deficient in TLR2, TLR4 or TLR2/4 to test the involvement of TLR signaling. Results: Intestinal epithelial cells are able to synthesize and release IL-10 and their expression is increased after TLR2 or TLR4 activation. IL-10 regulation seems to be tissue specific, with IL-10 expression in the ileum regulated by a compensation between TLR2 and TLR4 expression, whereas in the colon, TLR2 and TLR4 affect IL-10 expression independently. Conclusions: Intestinal epithelial cells could release IL-10 in response to TLR activation, playing an intestinal tissue-dependent and critical intestinal immune role.

Idioma: Inglés

DOI: 10.1177/2050640617727180

Año: 2018

Publicado en: United European Gastroenterology Journal 6, 3 (2018), 446-453
ISSN: 2050-6406

Factor impacto JCR: 3.453 (2018)

Categ. JCR: GASTROENTEROLOGY & HEPATOLOGY rank: 33 / 84 = 0.393 (2018) - Q2 - T2

Factor impacto SCIMAGO: 1.329 - Oncology (Q1) - Gastroenterology (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/B61
Financiación: info:eu-repo/grantAgreement/ES/MICINN-FEDER/BFU2010-18971
Financiación: info:eu-repo/grantAgreement/ES/UZ/2014-BIO-03

Tipo y forma: Article (Published version)

Área (Departamento): Área Fisiología (Dpto. Farmacología y Fisiolog.)
Área (Departamento): Área Inmunología (Dpto. Microb.Med.Pr.,Sal.Públ.)
Área (Departamento): Area Medicina (Dpto. Medicina, Psiqu. y Derm.)
Área (Departamento): Área Biología Celular (Dpto. Bioq.Biolog.Mol. Celular)