Oxidative stress in E. coli cells upon exposure to heat treatments

Marcén, M. (Universidad de Zaragoza) ; Ruiz, V. ; Serrano, M. J. ; Condón, S. (Universidad de Zaragoza) ; Mañas, P. (Universidad de Zaragoza)

Resumen: Heat treatments are widely used by the food industry to inactivate microorganisms, however their mode of action on microbial cells is not fully known. In the last years, it has been proposed that the generation of oxidative species could be an important factor contributing to cell death by heat and by other stresses; however, investigations in this field are scarce. The present work studies the generation of reactive oxygen species (ROS) upon heat treatment in E. coli, through the use of cell staining with specific fluorochromes. Results obtained demonstrate that ROS are detected in E. coli cells when they are subjected to heat exposure, and the amount of fluorescence increases with temperature and time, as does the cellular inactivation. The addition of glutathione or tiron, a potent antioxidant and a superoxide quencher, respectively, to the heating medium protected E. coli against heat inactivation and concurrently reduced the detection of ROS, especially in the case of glutathione. Finally, recovery of heated cells under conditions that relief oxidative stress produced an increase in cell survival. Data presented in this work support the view that ROS generation and subsequent control in bacterial cells could be an essential factor determining inactivation and survival upon exposure to heat, and it could be a potential target to increase the efficacy of current treatments.

Idioma: Inglés

DOI: 10.1016/j.ijfoodmicro.2016.10.023

Año: 2017

Publicado en: INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY 241 (2017), 198-205
ISSN: 0168-1605

Factor impacto JCR: 3.451 (2017)

Categ. JCR: FOOD SCIENCE & TECHNOLOGY rank: 17 / 133 = 0.128 (2017) - Q1 - T1
Categ. JCR: MICROBIOLOGY rank: 43 / 125 = 0.344 (2017) - Q2 - T2

Factor impacto SCIMAGO: 1.366 - Food Science (Q1) - Safety, Risk, Reliability and Quality (Q1) - Medicine (miscellaneous) (Q1) - Microbiology (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/C093-2014
Financiación: info:eu-repo/grantAgreement/ES/MINECO/AGL2012-33522
Financiación: info:eu-repo/grantAgreement/ES/MINECO/AGL2015-69565-P

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Tecnología de Alimentos (Dpto. Produc.Animal Cienc.Ali.)