The photosynthetic cytochrome c550 from the diatom Phaeodactylum tricornutum

Bernal-Bayard, P. ; Puerto-Galán, L. ; Yruela, I. (Universidad de Zaragoza) ; García-Rubio, I. ; Castell, C. ; Ortega, J. M. ; Alonso, P. J. (Universidad de Zaragoza) ; Roncel, M. ; Martínez, J. I. ; Hervás, M. ; Navarro, J. A.

Resumen: The photosynthetic cytochrome c550 from the marine diatom Phaeodactylum tricornutum has been purified and characterized. Cytochrome c550 is mostly obtained from the soluble cell extract in relatively large amounts. In addition, the protein appeared to be truncated in the last hydrophobic residues of the C-terminus, both in the soluble cytochrome c550 and in the protein extracted from the membrane fraction, as deduced by mass spectrometry analysis and the comparison with the gene sequence. Interestingly, it has been described that the C-terminus of cytochrome c550 forms a hydrophobic finger involved in the interaction with photosystem II in cyanobacteria. Cytochrome c550 was almost absent in solubilized photosystem II complex samples, in contrast with the PsbO and Psb31 extrinsic subunits, thus suggesting a lower affinity of cytochrome c550 for the photosystem II complex. Under iron-limiting conditions the amount of cytochrome c550 decreases up to about 45% as compared to iron-replete cells, pointing to an iron-regulated synthesis. Oxidized cytochrome c550 has been characterized using continuous wave EPR and pulse techniques, including HYSCORE, and the obtained results have been interpreted in terms of the electrostatic charge distribution in the surroundings of the heme centre.

Idioma: Inglés

DOI: 10.1007/s11120-016-0327-x

Año: 2017

Publicado en: PHOTOSYNTHESIS RESEARCH 133, 1-3 (2017), 273-287
ISSN: 0166-8595

Factor impacto JCR: 3.091 (2017)

Categ. JCR: PLANT SCIENCES rank: 39 / 222 = 0.176 (2017) - Q1 - T1

Factor impacto SCIMAGO: 1.359 - Medicine (miscellaneous) (Q1) - Plant Science (Q1) - Biochemistry (Q2) - Cell Biology (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/B18
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BIO2012-35271
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BIO2015-64169-P
Financiación: info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-64486-R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/MAT2011-23861

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Física Materia Condensada (Dpto. Física Materia Condensa.)