Toward an integrative and predictive sperm quality analysis in Bos taurus

Yániz, J.L. (Universidad de Zaragoza) ; Soler, C. ; Alquézar-Baeta, C. (Universidad de Zaragoza) ; Santolaria, P. (Universidad de Zaragoza)

Resumen: There is a need to develop more integrative sperm quality analysis methods, enabling researchers to evaluate different parameters simultaneously cell by cell. In this work, we present a new multi-parametric fluorescent test able to discriminate different sperm subpopulations based on their labeling pattern and motility characteristics. Cryopreserved semen samples from 20 Holstein bulls were used in the study. Analyses of sperm motility using computer-assisted sperm analysis (CASA-mot), membrane integrity by acridine orange-propidium iodide combination and multi-parametric by the ISAS®3Fun kit, were performed. The new method allows a clear discrimination of sperm subpopulations based on membrane and acrosomal integrity, motility and morphology. It was also possible to observe live spermatozoa showing signs of capacitation such as hyperactivated motility and changes in acrosomal structure. Sperm subpopulation with intact plasma membrane and acrosome showed a higher proportion of motile sperm than those with damaged acrosome or increased fluorescence intensity. Spermatozoa with intact plasmalemma and damaged acrosome were static or exhibit weak movement. Significant correlations among the different sperm quality parameters evaluated were also described. We concluded that the ISAS®3Fun is an integrated method that represents an advance in sperm quality analysis with the potential to improve fertility predictions.

Idioma: Inglés

DOI: 10.1016/j.anireprosci.2017.03.022

Año: 2017

Publicado en: ANIMAL REPRODUCTION SCIENCE 181 (2017), 108-114
ISSN: 0378-4320

Factor impacto JCR: 1.647 (2017)

Categ. JCR: AGRICULTURE, DAIRY & ANIMAL SCIENCE rank: 14 / 59 = 0.237 (2017) - Q1 - T1
Categ. JCR: REPRODUCTIVE BIOLOGY rank: 22 / 29 = 0.759 (2017) - Q4 - T3

Factor impacto SCIMAGO: 0.704 - Animal Science and Zoology (Q1) - Food Animals (Q1) - Medicine (miscellaneous) (Q2) - Endocrinology (Q3)

Financiación: info:eu-repo/grantAgreement/ES/DGA-FSE/A40
Financiación: info:eu-repo/grantAgreement/ES/MINECO/AGL2014-52775-P

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Área Zoología (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)