Graphene oxide functional nanohybrids with magnetic nanoparticles for improved vectorization of doxorubicin to neuroblastoma cells

Lerra, L. ; Farfalla, A. ; Sanz, B. (Universidad de Zaragoza) ; Cirillo, G. ; Vittorio, O. ; Voli, F. ; Grand, M.L. ; Curcio, M. ; Nicoletta, F.P. ; Dubrovska, A. ; Hampel, S. ; Iemma, F. ; Goya, G.F. (Universidad de Zaragoza)

Resumen: With the aim to obtain a site-specific doxorubicin (DOX) delivery in neuroblastoma SH-SY5Y cells, we designed an hybrid nanocarrier combining graphene oxide (GO) and magnetic iron oxide nanoparticles (MNPs), acting as core elements, and a curcumin–human serum albumin conjugate as functional coating. The nanohybrid, synthesized by redox reaction between the MNPs@GO system and albumin bioconjugate, consisted of MNPs@GO nanosheets homogeneously coated by the bioconjugate as verified by SEM investigations. Drug release experiments showed a pH-responsive behavior with higher release amounts in acidic (45% at pH 5.0) vs. neutral (28% at pH 7.4) environments. Cell internalization studies proved the presence of nanohybrid inside SH-SY5Y cytoplasm. The improved efficacy obtained in viability assays is given by the synergy of functional coating and MNPs constituting the nanohybrids: while curcumin moieties were able to keep low DOX cytotoxicity levels (at concentrations of 0.44–0.88 µM), the presence of MNPs allowed remote actuation on the nanohybrid by a magnetic field, increasing the dose delivered at the target site.

Idioma: Inglés

DOI: 10.3390/pharmaceutics11010003

Año: 2019

Publicado en: Pharmaceutics 11, 1 (2019), 3 [17 pp]
ISSN: 1999-4923

Factor impacto JCR: 4.421 (2019)

Categ. JCR: PHARMACOLOGY & PHARMACY rank: 44 / 270 = 0.163 (2019) - Q1 - T1

Factor impacto SCIMAGO: 0.894 - Pharmaceutical Science (Q1)

Tipo y forma: Article (Published version)

Área (Departamento): Área Física Materia Condensada (Dpto. Física Materia Condensa.)