Sub-Inhibitory doses of individual constituents of essential oils can select for staphylococcus aureus resistant mutants

Berdejo, D. (Universidad de Zaragoza) ; Chueca, B. ; Pagán, E. (Universidad de Zaragoza) ; Renzoni, A. ; Kelley, W.L. ; Pagán, R. (Universidad de Zaragoza) ; Garcia-Gonzalo, D. (Universidad de Zaragoza)

Resumen: Increased bacterial resistance to food preservation technologies represents a risk for food safety and shelf-life. The use of natural antimicrobials, such as essential oils (EOs) and their individual constituents (ICs), has been proposed to avoid the generation of antimicrobial resistance. However, prolonged application of ICs might conceivably lead to the emergence of resistant strains. Hence, this study was aimed toward applying sub-inhibitory doses of the ICs carvacrol, citral, and (+)-limonene oxide to Staphylococcus aureus USA300, in order to evaluate the emergence of resistant strains and to identify the genetic modifications responsible for their increased resistance. Three stable-resistant strains, CAR (from cultures with carvacrol), CIT (from cultures with citral), and OXLIM (from cultures with (+)-limonene oxide) were isolated, showing an increased resistance against the ICs and a higher tolerance to lethal treatments by ICs or heat. Whole-genome sequencing revealed in CAR a large deletion in a region that contained genes encoding transcriptional regulators and metabolic enzymes. CIT showed a single missense mutation in aroC (N187K), which encodes for chorismate synthase; and in OXLIM a missense mutation was detected in rpoB (A862V), which encodes for RNA polymerase subunit beta. This study provides a first detailed insight into the mechanisms of action and S. aureus resistance arising from exposure to carvacrol, citral, and (+)-limonene oxide.

Idioma: Inglés

DOI: 10.3390/molecules24010170

Año: 2019

Publicado en: Molecules 24, 1 (2019), 170 [16 pp]
ISSN: 1420-3049

Factor impacto JCR: 3.267 (2019)

Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 70 / 177 = 0.395 (2019) - Q2 - T2
Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 141 / 297 = 0.475 (2019) - Q2 - T2

Factor impacto SCIMAGO: 0.698 - Pharmaceutical Science (Q1) - Chemistry (miscellaneous) (Q2) - Drug Discovery (Q2) - Physical and Theoretical Chemistry (Q2) - Organic Chemistry (Q2) - Analytical Chemistry (Q2) - Medicine (miscellaneous) (Q2) - Molecular Medicine (Q3)

Financiación: info:eu-repo/grantAgreement/ES/MEC/FPU12-00170
Financiación: info:eu-repo/grantAgreement/ES/MEC/FPU15-02703
Financiación: info:eu-repo/grantAgreement/ES/MEC/FPU17-02441
Financiación: info:eu-repo/grantAgreement/ES/MINECO/AGL2015-69565-P

Tipo y forma: Article (Published version)

Área (Departamento): Área Tecnología de Alimentos (Dpto. Produc.Animal Cienc.Ali.)