Chitosan nanoparticles for combined drug delivery and magnetic hyperthermia: from preparation to in vitro studies

Zamora-Mora, Vanessa ; Fernández-Gutiérrez, Mar ; González-Gómez, Álvaro ; Sanz, Beatriz ; San Román, Julio ; Goya, Gerardo F. (Universidad de Zaragoza) ; Hernández, Rebeca ; Mijangos, Carmen

Resumen: Chitosan nanoparticles (CSNPs) ionically crosslinked with tripolyphosphate salts (TPP) were employed as nanocarriers in combined drug delivery and magnetic hyperthermia (MH) therapy. To that aim, three different ferrofluid concentrations and a constant 5-fluorouracil (5-FU) concentration were efficiently encapsulated to yield magnetic CSNPs with core-shell morphology. In vitro experiments using normal cells, fibroblasts (FHB) and cancer cells, human glioblastoma A-172, showed that CSNPs presented a dose-dependent cytotoxicity and that they were successfully uptaken into both cell lines. The application of a MH treatment in A-172 cells resulted in a cell viability of 67–75% whereas no significant reduction of cell viability was observed for FHB. However, the A-172 cells showed re-growth populations 4 h after the application of the MH treatment when CSNPs were loaded only with ferrofluid. Finally, a combined effect of MH and 5-FU release was observed with the application of a second MH treatment for CSNPs exhibiting a lower amount of released 5-FU. This result demonstrates the potential of CSNPs for the improvement of MH therapies.

Idioma: Inglés

DOI: 10.1016/j.carbpol.2016.09.084

Año: 2017

Publicado en: CARBOHYDRATE POLYMERS 157 (2017), 361-370 [34 p.]
ISSN: 0144-8617

Factor impacto JCR: 5.158 (2017)

Categ. JCR: CHEMISTRY, APPLIED rank: 2 / 71 = 0.028 (2017) - Q1 - T1
Categ. JCR: POLYMER SCIENCE rank: 7 / 87 = 0.08 (2017) - Q1 - T1
Categ. JCR: CHEMISTRY, ORGANIC rank: 6 / 57 = 0.105 (2017) - Q1 - T1

Factor impacto SCIMAGO: 1.428 - Materials Chemistry (Q1) - Polymers and Plastics (Q1) - Organic Chemistry (Q1)

Financiación: info:eu-repo/grantAgreement/ES/MINECO/MAT2010-19326
Financiación: info:eu-repo/grantAgreement/ES/MINECO/MAT2011-24797

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Física Materia Condensada (Dpto. Física Materia Condensa.)