Enabling cell recovery from 3D cell culture microfluidic devices for tumour microenvironment biomarker profiling

Virumbrales-Muñoz, M. ; Ayuso, J.M. ; Lacueva, A. (Universidad de Zaragoza) ; Randelovic, T. ; Livingston, M.K. ; Beebe, D.J. ; Oliván, S. (Universidad de Zaragoza) ; Pereboom, D. (Universidad de Zaragoza) ; Doblare, M. (Universidad de Zaragoza) ; Fernández, L. (Universidad de Zaragoza) ; Ochoa, I. (Universidad de Zaragoza)

Resumen: The tumour microenvironment (TME) has recently drawn much attention due to its profound impact on tumour development, drug resistance and patient outcome. There is an increasing interest in new therapies that target the TME. Nonetheless, most established in vitro models fail to include essential cues of the TME. Microfluidics can be used to reproduce the TME in vitro and hence provide valuable insight on tumour evolution and drug sensitivity. However, microfluidics remains far from well-established mainstream molecular and cell biology methods. Therefore, we have developed a quick and straightforward collagenase-based enzymatic method to recover cells embedded in a 3D hydrogel in a microfluidic device with no impact on cell viability. We demonstrate the validity of this method on two different cell lines in a TME microfluidic model. Cells were successfully retrieved with high viability, and we characterised the different cell death mechanisms via AMNIS image cytometry in our model.

Idioma: Inglés

DOI: 10.1038/s41598-019-42529-8

Año: 2019

Publicado en: Scientific reports (Nature Publishing Group) 9, 1 (2019), 6199 [14 pp]
ISSN: 2045-2322

Factor impacto JCR: 3.998 (2019)

Categ. JCR: MULTIDISCIPLINARY SCIENCES rank: 17 / 71 = 0.239 (2019) - Q1 - T1

Factor impacto SCIMAGO: 1.341 - Multidisciplinary (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/T24-17R
Financiación: info:eu-repo/grantAgreement/EC/H2020/766884/EU/Organ on Chip in Development/ORCHID
Financiación: info:eu-repo/grantAgreement/EC/H2020/778354/EU/Heart On chip based on induced pluripotent Stem cell Technology for personalized Medicine/CISTEM
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BIO2016-79092-R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/DPI2015-65401-C3-1-R

Tipo y forma: Article (Published version)

Área (Departamento): Área Fisiología (Dpto. Farmacología y Fisiolog.)
Área (Departamento): Area Histología (Dpto. Anatom.Histolog.Humanas)
Área (Departamento): Área Mec.Med.Cont. y Teor.Est. (Dpto. Ingeniería Mecánica)