ß-Carotene, a-tocoferol and rosmarinic acid encapsulated within PLA/PLGA microcarriers by supercritical emulsion extraction: Encapsulation efficiency, drugs shelf-life and antioxidant activity

Gimenez-Rota, C. ; Palazzo, I. ; Scognamiglio, M.R. ; Mainar, A. (Universidad de Zaragoza) ; Reverchon, E. ; Della Porta, G.

Resumen: ß-Carotene (ß-CA) is largely used antioxidant with a high sensibility to oxidation when in contact with light and temperature. To improve its shelf life and develop a nutraceutical formulation it was encapsulated into poly-lactic-co-glycolic acid (PLGA) and poly-lactic acid (PLA) carriers using Supercritical Emulsion Extraction (SEE). a-Tocoferol (a-TOC) and Rosmarinic Acid (RA) were proposed as excipients to improve product shelf-life. Different emulsion formulation conditions, such as compositions and mixing rate were used; whereas, SEE operating conditions in the counter-current tower were fixed at 80 bar and 38 °C with an L/G ratio of 0.1. In these conditions, PLA and PLGA carriers with sizes ranges between 1.5 ± 0.5 µm and 0.3 ± 0.1 µm were fabricated with Encapsulation Efficiencies (EEs) between 50–80%. The co-encapsulation of a-TOC with ß-CA gained to prolonged drug shelf life; whereas, RA co-encapsulation was not successfully and, in some cases, also reduced ß-CA-EE. The poor loading experienced, in the case of RA, was probably due to its high solubility into the high-pressure mixture of carbon dioxide and organic solvent formed during the emulsion extraction. This behaviour was defined “co-extraction effect” and may limit the application of SEE technology in the encapsulation of molecules soluble in the mixture obtained during oily phase extraction. Shelf-life studies were performed after UV irradiation for ten days and after carriers storage for 2 years at 4 °C. The better performance in terms of shelf life was observed for PLGA capsules loaded with ß-CA/a-TOC; whereas, PLA formulation with ß-CA/a-TOC showed a superior antioxidant activity with an half minimal Inhibitory Concentration (IC50) against the free radical DPPH of 1.8 mg/mL of carriers.

Idioma: Inglés

DOI: 10.1016/j.supflu.2019.01.019

Año: 2019

Publicado en: Journal of Supercritical Fluids 146 (2019), 199-207
ISSN: 0896-8446

Factor impacto JCR: 3.744 (2019)

Categ. JCR: ENGINEERING, CHEMICAL rank: 39 / 143 = 0.273 (2019) - Q2 - T1
Categ. JCR: CHEMISTRY, PHYSICAL rank: 60 / 158 = 0.38 (2019) - Q2 - T2

Factor impacto SCIMAGO: 1.055 - Chemical Engineering (miscellaneous) (Q1) - Physical and Theoretical Chemistry (Q1) - Condensed Matter Physics (Q1)

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Química Física (Dpto. Química Física)