2-oxoglutarate modulates the affinity of FurA for the ntcA promoter in Anabaena sp. PCC 7120

Guio, Jorge (Universidad de Zaragoza) ; Sarasa-Buisán, Cristina (Universidad de Zaragoza) ; Velázquez-Campoy, Adrián (Universidad de Zaragoza) ; Bes, María Teresa (Universidad de Zaragoza) ; Fillat, María F (Universidad de Zaragoza) ; Peleato, María Luisa (Universidad de Zaragoza) ; Sevilla, Emma (Universidad de Zaragoza)

Resumen: 2-oxoglutarate (2-OG) is a central metabolite that acts as a signaling molecule informing about the status of the carbon/nitrogen balance of the cell. In recent years, some transcriptional regulators and even two-component systems have been described as 2-OG sensors. In the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120, two master regulators, NtcA and FurA, are deeply involved in the regulation of nitrogen metabolism. Both of them show a complex intertwined regulatory circuit to achieve a suitable regulation of nitrogen fixation. In this work, 2-OG is found to bind FurA, modulating the specific binding of FurA to the ntcA promoter. This study provides evidence of a new additional control point in the complex network controlled by the NtcA and FurA proteins.

Idioma: Inglés

DOI: 10.1002/1873-3468.13610

Año: 2019

Publicado en: FEBS Letters 594, 2 (2019), 278-289
ISSN: 0014-5793

Factor impacto JCR: 3.057 (2019)

Categ. JCR: BIOPHYSICS rank: 24 / 71 = 0.338 (2019) - Q2 - T2
Categ. JCR: CELL BIOLOGY rank: 122 / 195 = 0.626 (2019) - Q3 - T2
Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 154 / 297 = 0.519 (2019) - Q3 - T2

Factor impacto SCIMAGO: 1.948 - Biochemistry (Q1) - Biophysics (Q1) - Structural Biology (Q1) - Genetics (Q1) - Molecular Biology (Q1) - Cell Biology (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/E35-17R
Financiación: info:eu-repo/grantAgreement/ES/MINECO/BFU2016-77671-P

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Fisiología Vegetal (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)