Genetic Variants and Phenotypic Characteristics ofSalmonellaTyphimurium-Resistant Mutants after Exposure to Carvacrol

Berdejo, D. (Universidad de Zaragoza) ; Merino, N. (Universidad de Zaragoza) ; Pagan, E. (Universidad de Zaragoza) ; Garcia-Gonzalo, D. (Universidad de Zaragoza) ; Pagan, R. (Universidad de Zaragoza)

Resumen: The emergence of antimicrobial resistance has raised questions about the safety of essential oils and their individual constituents as food preservatives and as disinfection agents. Further research is required to understand how and under what conditions stable genotypic resistance might occur in food pathogens. Evolution experiments onSalmonellaTyphimurium cyclically exposed to sublethal and lethal doses of carvacrol permitted the isolation of SeSCar and SeLCar strains, respectively. Both evolved strains showed a significant increase in carvacrol resistance, assessed by minimum inhibitory and bactericidal concentrations, the study of growth kinetics in the presence of carvacrol, and the evaluation of survival under lethal conditions. Moreover, antibiotic susceptibility tests revealed a development of SeLCar resistance to a wide range of antibiotics. Whole genome sequencing allowed the identification of single nucleotide variations in transcriptional regulators of oxidative stress-response:yfhPin SeSCar andsoxRin SeLCar, which could be responsible for the increased resistance by improving the response to carvacrol and preventing its accumulation inside the cell. This study demonstrates the emergence of S. Typhimurium-resistant mutants against carvacrol, which might pose a risk to food safety and should therefore be considered in the design of food preservation strategies, or of cleaning and disinfection treatments.

Idioma: Inglés

DOI: 10.3390/microorganisms8060937

Año: 2020

Publicado en: Microorganisms 8, 6 (2020), 937 [22 pp]
ISSN: 2076-2607

Factor impacto JCR: 4.128 (2020)

Categ. JCR: MICROBIOLOGY rank: 52 / 135 = 0.385 (2020) - Q2 - T2

Factor impacto SCIMAGO: 0.857 - Microbiology (Q2) - Virology (Q2) - Microbiology (medical) (Q2)

Financiación: info:eu-repo/grantAgreement/ES/AEI-ESF-DGA/PGC2018-093789-B-I00

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Tecnología de Alimentos (Dpto. Produc.Animal Cienc.Ali.)